Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31839 | 5' | -61.1 | NC_006938.1 | + | 52758 | 0.67 | 0.428067 |
Target: 5'- gCCCGGAacggcaGGGCGcGugGCUuccGGUUCa -3' miRNA: 3'- gGGGUCUg-----CCCGCuCugCGA---CCAGGc -5' |
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31839 | 5' | -61.1 | NC_006938.1 | + | 55023 | 0.66 | 0.464827 |
Target: 5'- aCCCGGAaGGGgauCGAcuGCGCUGGUCUc -3' miRNA: 3'- gGGGUCUgCCC---GCUc-UGCGACCAGGc -5' |
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31839 | 5' | -61.1 | NC_006938.1 | + | 55895 | 0.73 | 0.174837 |
Target: 5'- gCCCAGACaGGCGAGAgGgUGGggCCu -3' miRNA: 3'- gGGGUCUGcCCGCUCUgCgACCa-GGc -5' |
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31839 | 5' | -61.1 | NC_006938.1 | + | 57963 | 0.67 | 0.45548 |
Target: 5'- uUCCAgGACGGGUGGGACgGC-GGcUCCc -3' miRNA: 3'- gGGGU-CUGCCCGCUCUG-CGaCC-AGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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