Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31839 | 5' | -61.1 | NC_006938.1 | + | 12981 | 0.66 | 0.483813 |
Target: 5'- gCCCaaggAGGCGGGaGAGgccccggcgaGCGcCUGGUCCa -3' miRNA: 3'- -GGGg---UCUGCCCgCUC----------UGC-GACCAGGc -5' |
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31839 | 5' | -61.1 | NC_006938.1 | + | 8087 | 0.81 | 0.050039 |
Target: 5'- cUCCCAuGACGGGCGGGA-GCUGGgCCGg -3' miRNA: 3'- -GGGGU-CUGCCCGCUCUgCGACCaGGC- -5' |
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31839 | 5' | -61.1 | NC_006938.1 | + | 7488 | 0.7 | 0.292921 |
Target: 5'- gCCCUgAGcCGGGUcgacGAGugGCUGGcaUCCGg -3' miRNA: 3'- -GGGG-UCuGCCCG----CUCugCGACC--AGGC- -5' |
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31839 | 5' | -61.1 | NC_006938.1 | + | 7340 | 0.65 | 0.519873 |
Target: 5'- aUCCCGGACGagagaccguccuGGCucaguucgacgaaaGGGA-GCUGGUCCa -3' miRNA: 3'- -GGGGUCUGC------------CCG--------------CUCUgCGACCAGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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