miRNA: 3'- ggGGU-CuGCCCGc--UCugCGACCAGGC- -5'
miRNA: 3'- gGGGUCUGcCCGCUCUgCgACCa-GGc -5'
miRNA: 3'- -GGGGU-CUGCCCGCUCUgCGACCaGGC- -5'
miRNA: 3'- -GGGGUCUGCCCGCUCUGCGACCAGGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31839 | 5' | -61.1 | NC_006938.1 | + | 19817 | 0.72 | 0.230289 |
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Target: 5'- -aCCGcGuCGGGCagcGGugGCUGGUCCGg -3' miRNA: 3'- ggGGU-CuGCCCGc--UCugCGACCAGGC- -5' |
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| 31839 | 5' | -61.1 | NC_006938.1 | + | 55895 | 0.73 | 0.174837 |
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Target: 5'- gCCCAGACaGGCGAGAgGgUGGggCCu -3' miRNA: 3'- gGGGUCUGcCCGCUCUgCgACCa-GGc -5' |
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| 31839 | 5' | -61.1 | NC_006938.1 | + | 8087 | 0.81 | 0.050039 |
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Target: 5'- cUCCCAuGACGGGCGGGA-GCUGGgCCGg -3' miRNA: 3'- -GGGGU-CUGCCCGCUCUgCGACCaGGC- -5' |
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| 31839 | 5' | -61.1 | NC_006938.1 | + | 27327 | 1.09 | 0.000446 |
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Target: 5'- uCCCCAGACGGGCGAGACGCUGGUCCGc -3' miRNA: 3'- -GGGGUCUGCCCGCUCUGCGACCAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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