Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31840 | 3' | -58.9 | NC_006938.1 | + | 11244 | 0.66 | 0.506234 |
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Target: 5'- --gGCGGUGGCgauGAUGUgcccaagguGGGGGAc- -3' miRNA: 3'- aagCGCCACCGag-CUACG---------UCCCCUac -5' |
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| 31840 | 3' | -58.9 | NC_006938.1 | + | 32840 | 0.67 | 0.485975 |
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Target: 5'- -cCGC-GUGGCUCGAggGCAGcuGGGAc- -3' miRNA: 3'- aaGCGcCACCGAGCUa-CGUC--CCCUac -5' |
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| 31840 | 3' | -58.9 | NC_006938.1 | + | 33987 | 0.67 | 0.46611 |
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Target: 5'- cUCGCGGaggGGCUCcauGGUgGCAGGGagcucGAUGa -3' miRNA: 3'- aAGCGCCa--CCGAG---CUA-CGUCCC-----CUAC- -5' |
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| 31840 | 3' | -58.9 | NC_006938.1 | + | 42606 | 0.67 | 0.46611 |
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Target: 5'- --gGCGGUGGCgUCGAUGgAGuucgaGGGAa- -3' miRNA: 3'- aagCGCCACCG-AGCUACgUC-----CCCUac -5' |
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| 31840 | 3' | -58.9 | NC_006938.1 | + | 32247 | 0.7 | 0.309356 |
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Target: 5'- cUUCGCGca-GUUCGucgGCAGGGGAUGg -3' miRNA: 3'- -AAGCGCcacCGAGCua-CGUCCCCUAC- -5' |
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| 31840 | 3' | -58.9 | NC_006938.1 | + | 27548 | 1.06 | 0.000695 |
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Target: 5'- gUUCGCGGUGGCUCGAUGCAGGGGAUGg -3' miRNA: 3'- -AAGCGCCACCGAGCUACGUCCCCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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