miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31840 3' -58.9 NC_006938.1 + 11244 0.66 0.506234
Target:  5'- --gGCGGUGGCgauGAUGUgcccaagguGGGGGAc- -3'
miRNA:   3'- aagCGCCACCGag-CUACG---------UCCCCUac -5'
31840 3' -58.9 NC_006938.1 + 32840 0.67 0.485975
Target:  5'- -cCGC-GUGGCUCGAggGCAGcuGGGAc- -3'
miRNA:   3'- aaGCGcCACCGAGCUa-CGUC--CCCUac -5'
31840 3' -58.9 NC_006938.1 + 33987 0.67 0.46611
Target:  5'- cUCGCGGaggGGCUCcauGGUgGCAGGGagcucGAUGa -3'
miRNA:   3'- aAGCGCCa--CCGAG---CUA-CGUCCC-----CUAC- -5'
31840 3' -58.9 NC_006938.1 + 42606 0.67 0.46611
Target:  5'- --gGCGGUGGCgUCGAUGgAGuucgaGGGAa- -3'
miRNA:   3'- aagCGCCACCG-AGCUACgUC-----CCCUac -5'
31840 3' -58.9 NC_006938.1 + 32247 0.7 0.309356
Target:  5'- cUUCGCGca-GUUCGucgGCAGGGGAUGg -3'
miRNA:   3'- -AAGCGCcacCGAGCua-CGUCCCCUAC- -5'
31840 3' -58.9 NC_006938.1 + 27548 1.06 0.000695
Target:  5'- gUUCGCGGUGGCUCGAUGCAGGGGAUGg -3'
miRNA:   3'- -AAGCGCCACCGAGCUACGUCCCCUAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.