Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31846 | 3' | -56.8 | NC_006938.1 | + | 42522 | 0.69 | 0.460691 |
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Target: 5'- gGCCGAGGGAGuGGCaGGcgucgcuccggccGCGuCGGCg -3' miRNA: 3'- aCGGUUUCCUCuCUGaCC-------------UGC-GCCGa -5' |
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| 31846 | 3' | -56.8 | NC_006938.1 | + | 45835 | 0.66 | 0.630096 |
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Target: 5'- cGCCGAuGGAGAGAC-GGuuGUcuugGGCUc -3' miRNA: 3'- aCGGUUuCCUCUCUGaCCugCG----CCGA- -5' |
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| 31846 | 3' | -56.8 | NC_006938.1 | + | 47848 | 0.66 | 0.662732 |
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Target: 5'- gGCCGGAGGAGAGGCcuccaGcACGaGGCc -3' miRNA: 3'- aCGGUUUCCUCUCUGa----CcUGCgCCGa -5' |
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| 31846 | 3' | -56.8 | NC_006938.1 | + | 52676 | 0.66 | 0.673568 |
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Target: 5'- gGcCCAGAcccguGGGGAGug-GGACGuCGGCUa -3' miRNA: 3'- aC-GGUUU-----CCUCUCugaCCUGC-GCCGA- -5' |
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| 31846 | 3' | -56.8 | NC_006938.1 | + | 52949 | 0.67 | 0.565109 |
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Target: 5'- aGCCcGAGGuguaccgcuugaAGAG-CUGGAaCGUGGCg -3' miRNA: 3'- aCGGuUUCC------------UCUCuGACCU-GCGCCGa -5' |
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| 31846 | 3' | -56.8 | NC_006938.1 | + | 53986 | 0.69 | 0.442166 |
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Target: 5'- cGCCucgGAGGGGucGAC-GGugGCGGUUg -3' miRNA: 3'- aCGGu--UUCCUCu-CUGaCCugCGCCGA- -5' |
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| 31846 | 3' | -56.8 | NC_006938.1 | + | 55613 | 0.66 | 0.619208 |
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Target: 5'- cGCCGAGacGGAGAGgucGCcGaGGCGUGGCc -3' miRNA: 3'- aCGGUUU--CCUCUC---UGaC-CUGCGCCGa -5' |
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| 31846 | 3' | -56.8 | NC_006938.1 | + | 58906 | 0.7 | 0.395519 |
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Target: 5'- cGCCAAcGGuGAG-CUuGACGCGGCc -3' miRNA: 3'- aCGGUUuCCuCUCuGAcCUGCGCCGa -5' |
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| 31846 | 3' | -56.8 | NC_006938.1 | + | 61133 | 0.7 | 0.395519 |
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Target: 5'- aUGCCAGAGGAcAGGuucUUGuACGCGGCg -3' miRNA: 3'- -ACGGUUUCCUcUCU---GACcUGCGCCGa -5' |
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| 31846 | 3' | -56.8 | NC_006938.1 | + | 62102 | 0.66 | 0.673568 |
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Target: 5'- cGCCAAcucuGGuccgcuuuGGAUUGGACGCGaGCg -3' miRNA: 3'- aCGGUUu---CCuc------UCUGACCUGCGC-CGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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