Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31854 | 5' | -52.6 | NC_006938.1 | + | 15105 | 0.66 | 0.856138 |
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Target: 5'- cGUACCUCCGggagUCaGGGCUGaCUCg-- -3' miRNA: 3'- -CGUGGAGGUaga-AG-CUCGAC-GAGaug -5' |
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| 31854 | 5' | -52.6 | NC_006938.1 | + | 2135 | 0.66 | 0.856138 |
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Target: 5'- gGCcCCUCgCGUCagcgUUGAGCUGC-CUGa -3' miRNA: 3'- -CGuGGAG-GUAGa---AGCUCGACGaGAUg -5' |
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| 31854 | 5' | -52.6 | NC_006938.1 | + | 52253 | 0.66 | 0.838664 |
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Target: 5'- cCGCCUCC-UCcacCGAGgaGCUgCUGCa -3' miRNA: 3'- cGUGGAGGuAGaa-GCUCgaCGA-GAUG- -5' |
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| 31854 | 5' | -52.6 | NC_006938.1 | + | 38418 | 0.67 | 0.82029 |
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Target: 5'- cGgGCCUUC-UCUUCaguGAGCUGCUggACg -3' miRNA: 3'- -CgUGGAGGuAGAAG---CUCGACGAgaUG- -5' |
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| 31854 | 5' | -52.6 | NC_006938.1 | + | 45912 | 0.67 | 0.801096 |
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Target: 5'- cGUcUCUCCAUCggCGGGCUGCg---- -3' miRNA: 3'- -CGuGGAGGUAGaaGCUCGACGagaug -5' |
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| 31854 | 5' | -52.6 | NC_006938.1 | + | 16663 | 0.68 | 0.781169 |
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Target: 5'- -aGCCUCCGagaucggcUCUUUGAGCaGCUCc-- -3' miRNA: 3'- cgUGGAGGU--------AGAAGCUCGaCGAGaug -5' |
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| 31854 | 5' | -52.6 | NC_006938.1 | + | 25786 | 0.68 | 0.7606 |
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Target: 5'- cCGCgCUCCGUCgUUGAGCUGUg--GCa -3' miRNA: 3'- cGUG-GAGGUAGaAGCUCGACGagaUG- -5' |
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| 31854 | 5' | -52.6 | NC_006938.1 | + | 24302 | 0.71 | 0.562235 |
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Target: 5'- aGCGCCUCCA----CGAGgUGCUCgACg -3' miRNA: 3'- -CGUGGAGGUagaaGCUCgACGAGaUG- -5' |
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| 31854 | 5' | -52.6 | NC_006938.1 | + | 54261 | 0.76 | 0.344629 |
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Target: 5'- cCACCUCCAUCUUUGAcGCgGCgucuguggUCUACa -3' miRNA: 3'- cGUGGAGGUAGAAGCU-CGaCG--------AGAUG- -5' |
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| 31854 | 5' | -52.6 | NC_006938.1 | + | 32176 | 1.11 | 0.001459 |
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Target: 5'- uGCACCUCCAUCUUCGAGCUGCUCUACc -3' miRNA: 3'- -CGUGGAGGUAGAAGCUCGACGAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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