Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31857 | 5' | -61.9 | NC_006938.1 | + | 8704 | 0.71 | 0.193859 |
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Target: 5'- uUCGGCGgcccucgugaugGCCGUGUagaGCCACCuGUUCg -3' miRNA: 3'- cAGCCGU------------UGGCACGg--CGGUGGcCGAG- -5' |
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| 31857 | 5' | -61.9 | NC_006938.1 | + | 30940 | 0.71 | 0.188958 |
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Target: 5'- cGUCGGCGGCCaGUGCC-CCAgagaccUCGGCg- -3' miRNA: 3'- -CAGCCGUUGG-CACGGcGGU------GGCCGag -5' |
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| 31857 | 5' | -61.9 | NC_006938.1 | + | 39585 | 0.71 | 0.179483 |
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Target: 5'- cUCGGaCGA-CGUcGCCGCCACUGGCa- -3' miRNA: 3'- cAGCC-GUUgGCA-CGGCGGUGGCCGag -5' |
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| 31857 | 5' | -61.9 | NC_006938.1 | + | 63447 | 0.73 | 0.134222 |
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Target: 5'- cGUgGGCGACCGgcucGCCGCCGCCcuucggaaccuucGGCa- -3' miRNA: 3'- -CAgCCGUUGGCa---CGGCGGUGG-------------CCGag -5' |
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| 31857 | 5' | -61.9 | NC_006938.1 | + | 46674 | 0.74 | 0.11591 |
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Target: 5'- cGUCGGCAgcuggggagcgcgggGCCGUGUCGCCucuCCagGGCUUc -3' miRNA: 3'- -CAGCCGU---------------UGGCACGGCGGu--GG--CCGAG- -5' |
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| 31857 | 5' | -61.9 | NC_006938.1 | + | 41392 | 0.75 | 0.102968 |
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Target: 5'- -cUGGCGucuguguugACCGUGgCGCCACCGGCcCg -3' miRNA: 3'- caGCCGU---------UGGCACgGCGGUGGCCGaG- -5' |
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| 31857 | 5' | -61.9 | NC_006938.1 | + | 14534 | 0.76 | 0.080621 |
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Target: 5'- --gGGaCGAgUGUGCCGCCAUUGGCUCg -3' miRNA: 3'- cagCC-GUUgGCACGGCGGUGGCCGAG- -5' |
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| 31857 | 5' | -61.9 | NC_006938.1 | + | 33575 | 1.09 | 0.000249 |
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Target: 5'- cGUCGGCAACCGUGCCGCCACCGGCUCa -3' miRNA: 3'- -CAGCCGUUGGCACGGCGGUGGCCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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