Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31861 | 3' | -56.8 | NC_006938.1 | + | 16690 | 0.66 | 0.661938 |
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Target: 5'- aAUGCauUCCuucuGGGUcCAGGGACgAGCCu -3' miRNA: 3'- gUACGacAGGc---UCCA-GUCCCUG-UCGG- -5' |
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| 31861 | 3' | -56.8 | NC_006938.1 | + | 16272 | 0.66 | 0.651157 |
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Target: 5'- aCGUGCcgGagCGcuGGGUCGGcGACAGCCc -3' miRNA: 3'- -GUACGa-CagGC--UCCAGUCcCUGUCGG- -5' |
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| 31861 | 3' | -56.8 | NC_006938.1 | + | 59138 | 0.67 | 0.597185 |
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Target: 5'- --aGCUGgCaaAGGUCAGGGugauucCAGCCg -3' miRNA: 3'- guaCGACaGgcUCCAGUCCCu-----GUCGG- -5' |
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| 31861 | 3' | -56.8 | NC_006938.1 | + | 30840 | 0.68 | 0.523021 |
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Target: 5'- --aGgaGgucgCCGAGGUCucuGGGGCAcugGCCg -3' miRNA: 3'- guaCgaCa---GGCUCCAGu--CCCUGU---CGG- -5' |
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| 31861 | 3' | -56.8 | NC_006938.1 | + | 20668 | 0.68 | 0.521984 |
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Target: 5'- -cUGCUGUuguggcgccgccgCCGuGGUgagaacCAGGGACAGgCCg -3' miRNA: 3'- guACGACA-------------GGCuCCA------GUCCCUGUC-GG- -5' |
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| 31861 | 3' | -56.8 | NC_006938.1 | + | 51912 | 0.7 | 0.414847 |
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Target: 5'- --cGCUGcagcagacgcUCUGGGGUCAGGcGGCGGUg -3' miRNA: 3'- guaCGAC----------AGGCUCCAGUCC-CUGUCGg -5' |
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| 31861 | 3' | -56.8 | NC_006938.1 | + | 35671 | 0.75 | 0.209772 |
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Target: 5'- gAUGCUGUCCGucGcCAGGGAgGaGCCc -3' miRNA: 3'- gUACGACAGGCucCaGUCCCUgU-CGG- -5' |
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| 31861 | 3' | -56.8 | NC_006938.1 | + | 28259 | 0.75 | 0.204368 |
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Target: 5'- aGUGCUGUgUGuAGGgCAGGGGCGGCa -3' miRNA: 3'- gUACGACAgGC-UCCaGUCCCUGUCGg -5' |
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| 31861 | 3' | -56.8 | NC_006938.1 | + | 6967 | 0.77 | 0.169379 |
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Target: 5'- --cGCUcugauccGUCCuAGGUCAGGGGCAGCg -3' miRNA: 3'- guaCGA-------CAGGcUCCAGUCCCUGUCGg -5' |
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| 31861 | 3' | -56.8 | NC_006938.1 | + | 37002 | 1.12 | 0.000538 |
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Target: 5'- uCAUGCUGUCCGAGGUCAGGGACAGCCg -3' miRNA: 3'- -GUACGACAGGCUCCAGUCCCUGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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