Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31861 | 5' | -60.1 | NC_006938.1 | + | 1524 | 0.68 | 0.399185 |
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Target: 5'- gCGGuGGCAgguagUGcACCCGGCCCgUGgcgACCg -3' miRNA: 3'- -GCC-CUGU-----AC-UGGGCCGGGgACag-UGG- -5' |
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| 31861 | 5' | -60.1 | NC_006938.1 | + | 27608 | 0.67 | 0.48207 |
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Target: 5'- gCGGGuGCGUGGCCCcGCaguCCUGgCAUCg -3' miRNA: 3'- -GCCC-UGUACUGGGcCGg--GGACaGUGG- -5' |
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| 31861 | 5' | -60.1 | NC_006938.1 | + | 36295 | 0.67 | 0.48207 |
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Target: 5'- gGGGuCGUGACCCGauggcGUCCCcGUCGa- -3' miRNA: 3'- gCCCuGUACUGGGC-----CGGGGaCAGUgg -5' |
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| 31861 | 5' | -60.1 | NC_006938.1 | + | 30098 | 0.66 | 0.501615 |
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Target: 5'- uGGcuCAUGagcuGCCaCGGCCaCCUGUCugCu -3' miRNA: 3'- gCCcuGUAC----UGG-GCCGG-GGACAGugG- -5' |
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| 31861 | 5' | -60.1 | NC_006938.1 | + | 15923 | 0.66 | 0.541685 |
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Target: 5'- aGGaACAUGACCaGGUCCUgGUC-CCa -3' miRNA: 3'- gCCcUGUACUGGgCCGGGGaCAGuGG- -5' |
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| 31861 | 5' | -60.1 | NC_006938.1 | + | 55980 | 0.66 | 0.541685 |
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Target: 5'- cCGGGcACcUGGCCgGaGCCCUcGcUCGCCu -3' miRNA: 3'- -GCCC-UGuACUGGgC-CGGGGaC-AGUGG- -5' |
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| 31861 | 5' | -60.1 | NC_006938.1 | + | 34508 | 0.66 | 0.550853 |
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Target: 5'- aGGGGCGgcUGGUCUGGCUCCggcacgaUGUCGCa -3' miRNA: 3'- gCCCUGU--ACUGGGCCGGGG-------ACAGUGg -5' |
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| 31861 | 5' | -60.1 | NC_006938.1 | + | 3933 | 0.71 | 0.276289 |
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Target: 5'- gGGGAUA-GACguugaaCGGCCCCUcGUCgACCg -3' miRNA: 3'- gCCCUGUaCUGg-----GCCGGGGA-CAG-UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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