Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31863 | 3' | -59.2 | NC_006938.1 | + | 51830 | 0.68 | 0.455519 |
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Target: 5'- uCGGUGaUCugUCCCcucugGGCAUCGaagCCCUc -3' miRNA: 3'- -GCCAC-AGugAGGG-----CCGUAGUg--GGGAc -5' |
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| 31863 | 3' | -59.2 | NC_006938.1 | + | 39066 | 0.69 | 0.399482 |
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Target: 5'- uGGUGUCGCUggcauguacgagaCCUGGCucuUCgGCCCCg- -3' miRNA: 3'- gCCACAGUGA-------------GGGCCGu--AG-UGGGGac -5' |
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| 31863 | 3' | -59.2 | NC_006938.1 | + | 49697 | 0.7 | 0.349659 |
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Target: 5'- aCGGagcgCGCUCCCGGUcuGUCAgucacaccgauCCCCUGg -3' miRNA: 3'- -GCCaca-GUGAGGGCCG--UAGU-----------GGGGAC- -5' |
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| 31863 | 3' | -59.2 | NC_006938.1 | + | 10718 | 0.71 | 0.269054 |
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Target: 5'- aCGGUGUCGggCgUGGuCAUCGCCCCg- -3' miRNA: 3'- -GCCACAGUgaGgGCC-GUAGUGGGGac -5' |
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| 31863 | 3' | -59.2 | NC_006938.1 | + | 12188 | 0.72 | 0.23767 |
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Target: 5'- gGGUGUCAC-CCUGGCugaCAUCCgCUGg -3' miRNA: 3'- gCCACAGUGaGGGCCGua-GUGGG-GAC- -5' |
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| 31863 | 3' | -59.2 | NC_006938.1 | + | 37836 | 1.08 | 0.000604 |
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Target: 5'- uCGGUGUCACUCCCGGCAUCACCCCUGa -3' miRNA: 3'- -GCCACAGUGAGGGCCGUAGUGGGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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