Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31866 | 3' | -51 | NC_006938.1 | + | 28958 | 0.66 | 0.924714 |
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Target: 5'- cGCAGUccgggUCGUC-GAuCUGC-ACGACUCg -3' miRNA: 3'- -CGUCA-----AGCAGaCUcGGCGuUGUUGAG- -5' |
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| 31866 | 3' | -51 | NC_006938.1 | + | 61917 | 0.66 | 0.918591 |
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Target: 5'- uGguGUUgaUCUGAGCCagGCuguCGACUCg -3' miRNA: 3'- -CguCAAgcAGACUCGG--CGuu-GUUGAG- -5' |
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| 31866 | 3' | -51 | NC_006938.1 | + | 21934 | 0.66 | 0.918591 |
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Target: 5'- cGCGaucacgUCGUCUGGGaucuCGCAGCGaaccaGCUCg -3' miRNA: 3'- -CGUca----AGCAGACUCg---GCGUUGU-----UGAG- -5' |
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| 31866 | 3' | -51 | NC_006938.1 | + | 56433 | 0.67 | 0.905478 |
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Target: 5'- aGCAGgaggGUCgGAGCCGCGA-AGCUg -3' miRNA: 3'- -CGUCaag-CAGaCUCGGCGUUgUUGAg -5' |
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| 31866 | 3' | -51 | NC_006938.1 | + | 37677 | 0.67 | 0.883678 |
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Target: 5'- aGCGGggcCGUCUGGGUgGCGAUggUg- -3' miRNA: 3'- -CGUCaa-GCAGACUCGgCGUUGuuGag -5' |
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| 31866 | 3' | -51 | NC_006938.1 | + | 15058 | 0.67 | 0.875861 |
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Target: 5'- gGCAGUUUGUCaacggGAGCCGgGAUguugAGCg- -3' miRNA: 3'- -CGUCAAGCAGa----CUCGGCgUUG----UUGag -5' |
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| 31866 | 3' | -51 | NC_006938.1 | + | 32252 | 0.68 | 0.86778 |
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Target: 5'- cGCAGUUCGUCggcaggggaugGGGCCuGCAguACAAg-- -3' miRNA: 3'- -CGUCAAGCAGa----------CUCGG-CGU--UGUUgag -5' |
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| 31866 | 3' | -51 | NC_006938.1 | + | 33569 | 0.69 | 0.804477 |
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Target: 5'- cCGGUUCGUCggcaaccGuGCCGCcacCGGCUCa -3' miRNA: 3'- cGUCAAGCAGa------CuCGGCGuu-GUUGAG- -5' |
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| 31866 | 3' | -51 | NC_006938.1 | + | 54521 | 0.71 | 0.676705 |
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Target: 5'- cCGGggUCGUCaGGGCCGCGAgGACa- -3' miRNA: 3'- cGUCa-AGCAGaCUCGGCGUUgUUGag -5' |
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| 31866 | 3' | -51 | NC_006938.1 | + | 38599 | 1.12 | 0.001963 |
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Target: 5'- cGCAGUUCGUCUGAGCCGCAACAACUCc -3' miRNA: 3'- -CGUCAAGCAGACUCGGCGUUGUUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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