Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31874 | 5' | -59.9 | NC_006938.1 | + | 25744 | 0.66 | 0.521996 |
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Target: 5'- -gCGAGGAgaacuaCgUCAUGUGCUCCgUGGu -3' miRNA: 3'- caGCUCCU------GgAGUACGCGAGGgGCCu -5' |
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| 31874 | 5' | -59.9 | NC_006938.1 | + | 17220 | 0.66 | 0.491957 |
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Target: 5'- -gCGAGGACCUUG-GUGacCUCCuuGGAc -3' miRNA: 3'- caGCUCCUGGAGUaCGC--GAGGggCCU- -5' |
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| 31874 | 5' | -59.9 | NC_006938.1 | + | 39386 | 0.69 | 0.364253 |
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Target: 5'- aUCGGGGAgCUCucGgGCUCCCCGc- -3' miRNA: 3'- cAGCUCCUgGAGuaCgCGAGGGGCcu -5' |
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| 31874 | 5' | -59.9 | NC_006938.1 | + | 2700 | 0.69 | 0.356046 |
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Target: 5'- -cCGAGGAUCUgGuacgacucguUGCGCUCCaggaaCCGGAc -3' miRNA: 3'- caGCUCCUGGAgU----------ACGCGAGG-----GGCCU- -5' |
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| 31874 | 5' | -59.9 | NC_006938.1 | + | 19544 | 0.69 | 0.356046 |
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Target: 5'- aUCGGGGACUgCGUGUGC-CUCgCGGAc -3' miRNA: 3'- cAGCUCCUGGaGUACGCGaGGG-GCCU- -5' |
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| 31874 | 5' | -59.9 | NC_006938.1 | + | 41157 | 0.69 | 0.337669 |
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Target: 5'- uGUCGAGGACCcggCcacggaggaggcgaGUGCGCUCCCg--- -3' miRNA: 3'- -CAGCUCCUGGa--G--------------UACGCGAGGGgccu -5' |
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| 31874 | 5' | -59.9 | NC_006938.1 | + | 49613 | 0.72 | 0.213975 |
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Target: 5'- aGUCGAGGACCUCGUuuaGUUUCCCaGGu -3' miRNA: 3'- -CAGCUCCUGGAGUAcg-CGAGGGG-CCu -5' |
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| 31874 | 5' | -59.9 | NC_006938.1 | + | 20045 | 0.74 | 0.169657 |
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Target: 5'- uUCGuGGGAcgguCCUCcagGCGCUCCCUGGAg -3' miRNA: 3'- cAGC-UCCU----GGAGua-CGCGAGGGGCCU- -5' |
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| 31874 | 5' | -59.9 | NC_006938.1 | + | 35591 | 0.76 | 0.126761 |
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Target: 5'- cGUCGAGGAUCUCGaagaUGCGCUggcgcaCCCGGc -3' miRNA: 3'- -CAGCUCCUGGAGU----ACGCGAg-----GGGCCu -5' |
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| 31874 | 5' | -59.9 | NC_006938.1 | + | 40593 | 1.08 | 0.000515 |
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Target: 5'- gGUCGAGGACCUCAUGCGCUCCCCGGAg -3' miRNA: 3'- -CAGCUCCUGGAGUACGCGAGGGGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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