Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31876 | 3' | -59.7 | NC_006938.1 | + | 63347 | 0.66 | 0.458174 |
Target: 5'- gAAGGUUCCGAagGGCGgCGGCGagcCGGu -3' miRNA: 3'- -UUCCGAGGCUagCUGC-GCCGCga-GUU- -5' |
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31876 | 3' | -59.7 | NC_006938.1 | + | 39364 | 0.67 | 0.438934 |
Target: 5'- uGAGGUUgUUGGUCGuCGCGGCGUUgGAc -3' miRNA: 3'- -UUCCGA-GGCUAGCuGCGCCGCGAgUU- -5' |
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31876 | 3' | -59.7 | NC_006938.1 | + | 56443 | 0.67 | 0.420165 |
Target: 5'- uGGGCUCC--UCGACGCGGacaGCa--- -3' miRNA: 3'- uUCCGAGGcuAGCUGCGCCg--CGaguu -5' |
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31876 | 3' | -59.7 | NC_006938.1 | + | 3828 | 0.7 | 0.281885 |
Target: 5'- gGAGGgaaCUCCGGUCGACGaggGGcCGUUCAAc -3' miRNA: 3'- -UUCC---GAGGCUAGCUGCg--CC-GCGAGUU- -5' |
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31876 | 3' | -59.7 | NC_006938.1 | + | 40899 | 0.93 | 0.005952 |
Target: 5'- cAAGGCUCCGAUCGACGCGG-GCUCAAu -3' miRNA: 3'- -UUCCGAGGCUAGCUGCGCCgCGAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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