Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31877 | 5' | -58.1 | NC_006938.1 | + | 40926 | 0.66 | 0.578703 |
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Target: 5'- cAGCGCaagcccaaGCUCggcUCGACCCGCcccGCGa -3' miRNA: 3'- cUCGCGg-------CGAGa--AGUUGGGCGa--CGUc -5' |
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| 31877 | 5' | -58.1 | NC_006938.1 | + | 52727 | 0.67 | 0.494866 |
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Target: 5'- -uGCGCCGCUCga-AACCCGaCgagagGguGa -3' miRNA: 3'- cuCGCGGCGAGaagUUGGGC-Ga----CguC- -5' |
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| 31877 | 5' | -58.1 | NC_006938.1 | + | 21602 | 0.67 | 0.474739 |
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Target: 5'- gGAGCuCuCGCUCUUCccacaGGCuCCGCUGCuGg -3' miRNA: 3'- -CUCGcG-GCGAGAAG-----UUG-GGCGACGuC- -5' |
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| 31877 | 5' | -58.1 | NC_006938.1 | + | 24258 | 0.69 | 0.398712 |
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Target: 5'- cGAcCGCCacauGgaCUUCGACCCGCUGCu- -3' miRNA: 3'- -CUcGCGG----CgaGAAGUUGGGCGACGuc -5' |
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| 31877 | 5' | -58.1 | NC_006938.1 | + | 32018 | 0.7 | 0.363734 |
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Target: 5'- uGAGCGCCaGCUCgagCAGCuuGUcagaguacuUGCGGa -3' miRNA: 3'- -CUCGCGG-CGAGaa-GUUGggCG---------ACGUC- -5' |
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| 31877 | 5' | -58.1 | NC_006938.1 | + | 41625 | 1.09 | 0.000564 |
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Target: 5'- gGAGCGCCGCUCUUCAACCCGCUGCAGg -3' miRNA: 3'- -CUCGCGGCGAGAAGUUGGGCGACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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