Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
31879 | 3' | -56.2 | NC_006938.1 | + | 48383 | 0.66 | 0.72392 |
Target: 5'- aCCgUCGGCACUGucuccacgauCGAGGCGGccacggCCUa- -3' miRNA: 3'- -GG-AGUCGUGAU----------GCUCCGCCaa----GGGag -5' |
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31879 | 3' | -56.2 | NC_006938.1 | + | 10269 | 0.66 | 0.713433 |
Target: 5'- uCCUCGGCcgugacCUGgucCGAGGUcuUUCCCUCg -3' miRNA: 3'- -GGAGUCGu-----GAU---GCUCCGccAAGGGAG- -5' |
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31879 | 3' | -56.2 | NC_006938.1 | + | 36249 | 0.66 | 0.713433 |
Target: 5'- aCUaCGGCAgUugaagugaaGAGGCGGgaucggUCCCUCc -3' miRNA: 3'- gGA-GUCGUgAug-------CUCCGCCa-----AGGGAG- -5' |
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31879 | 3' | -56.2 | NC_006938.1 | + | 12392 | 0.66 | 0.71238 |
Target: 5'- cCCUCggAGCGcCUGCGAuagaucuGGUGGcgcUCCCUUg -3' miRNA: 3'- -GGAG--UCGU-GAUGCU-------CCGCCa--AGGGAG- -5' |
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31879 | 3' | -56.2 | NC_006938.1 | + | 2568 | 0.66 | 0.689037 |
Target: 5'- cCCUgCAGCACgcCGGGGUcuccaggcagguccGGUUCCUg- -3' miRNA: 3'- -GGA-GUCGUGauGCUCCG--------------CCAAGGGag -5' |
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31879 | 3' | -56.2 | NC_006938.1 | + | 51843 | 0.66 | 0.68155 |
Target: 5'- cUCUCcguGGCACcaucgACGAGGUGGagaaCCUCa -3' miRNA: 3'- -GGAG---UCGUGa----UGCUCCGCCaag-GGAG- -5' |
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31879 | 3' | -56.2 | NC_006938.1 | + | 51358 | 0.68 | 0.563143 |
Target: 5'- aUUCGccGCGCUGC-AGGCGGUcuUCCCg- -3' miRNA: 3'- gGAGU--CGUGAUGcUCCGCCA--AGGGag -5' |
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31879 | 3' | -56.2 | NC_006938.1 | + | 42495 | 1.12 | 0.000654 |
Target: 5'- uCCUCAGCACUACGAGGCGGUUCCCUCg -3' miRNA: 3'- -GGAGUCGUGAUGCUCCGCCAAGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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