Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31880 | 5' | -50.9 | NC_006938.1 | + | 30545 | 0.66 | 0.914498 |
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Target: 5'- aUGGUGACUGGG-GAU--GCgGUGGGu -3' miRNA: 3'- -ACUACUGACCCaCUGguUGaUACCUu -5' |
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| 31880 | 5' | -50.9 | NC_006938.1 | + | 14616 | 0.67 | 0.886014 |
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Target: 5'- aGAUGGCUGGcUG-CCGACggucgGGAAc -3' miRNA: 3'- aCUACUGACCcACuGGUUGaua--CCUU- -5' |
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| 31880 | 5' | -50.9 | NC_006938.1 | + | 34430 | 0.67 | 0.87818 |
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Target: 5'- cGGUGGCUcGGcGUGACCAucuCUcccUGGGAg -3' miRNA: 3'- aCUACUGA-CC-CACUGGUu--GAu--ACCUU- -5' |
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| 31880 | 5' | -50.9 | NC_006938.1 | + | 7288 | 0.7 | 0.733041 |
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Target: 5'- cUGAUGACUGGGUacaGA-CAGCUGgucGGAc -3' miRNA: 3'- -ACUACUGACCCA---CUgGUUGAUa--CCUu -5' |
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| 31880 | 5' | -50.9 | NC_006938.1 | + | 43827 | 0.71 | 0.665776 |
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Target: 5'- cGGUGGCUGuGGUG-CgAGCUGUGGc- -3' miRNA: 3'- aCUACUGAC-CCACuGgUUGAUACCuu -5' |
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| 31880 | 5' | -50.9 | NC_006938.1 | + | 43569 | 1.06 | 0.004063 |
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Target: 5'- uUGAUGACUGGGUGACCAACUAUGGAAc -3' miRNA: 3'- -ACUACUGACCCACUGGUUGAUACCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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