Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31882 | 5' | -62.1 | NC_006938.1 | + | 10953 | 0.71 | 0.189885 |
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Target: 5'- gCCGCGUUCCCCugcacuuGCUGCCaACaucggccgggucuGGGGAg -3' miRNA: 3'- -GGCGCGAGGGGu------CGACGGcUG-------------CUCCU- -5' |
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| 31882 | 5' | -62.1 | NC_006938.1 | + | 13740 | 0.72 | 0.185607 |
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Target: 5'- gCCGCGCUCCaugagcaugUCAGCgcgGCCGAgUGAcGGAa -3' miRNA: 3'- -GGCGCGAGG---------GGUCGa--CGGCU-GCU-CCU- -5' |
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| 31882 | 5' | -62.1 | NC_006938.1 | + | 40210 | 0.72 | 0.185607 |
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Target: 5'- gCCGCGacaCCCGGCUGaCCGGCgcuguccgcgucGAGGAa -3' miRNA: 3'- -GGCGCgagGGGUCGAC-GGCUG------------CUCCU- -5' |
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| 31882 | 5' | -62.1 | NC_006938.1 | + | 4341 | 0.72 | 0.171957 |
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Target: 5'- aCCGCGCUCgCCgAGCUGCgaaGGCaGAcGGAc -3' miRNA: 3'- -GGCGCGAG-GGgUCGACGg--CUG-CU-CCU- -5' |
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| 31882 | 5' | -62.1 | NC_006938.1 | + | 52401 | 0.73 | 0.147322 |
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Target: 5'- gUCGaUGCcgCCCUGGCcGCCGAUGAGGAu -3' miRNA: 3'- -GGC-GCGa-GGGGUCGaCGGCUGCUCCU- -5' |
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| 31882 | 5' | -62.1 | NC_006938.1 | + | 58139 | 0.74 | 0.119481 |
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Target: 5'- -gGCGUUgaucgucacgCCCCAGUcugUGCCGGCGAGGGc -3' miRNA: 3'- ggCGCGA----------GGGGUCG---ACGGCUGCUCCU- -5' |
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| 31882 | 5' | -62.1 | NC_006938.1 | + | 20026 | 0.77 | 0.077909 |
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Target: 5'- -gGCGCUCCCUGGaguccuccacGCCGACGGGGAa -3' miRNA: 3'- ggCGCGAGGGGUCga--------CGGCUGCUCCU- -5' |
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| 31882 | 5' | -62.1 | NC_006938.1 | + | 41696 | 0.8 | 0.05039 |
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Target: 5'- gCGCGCUCCCCuGGCcgUGCCGACGuuGAu -3' miRNA: 3'- gGCGCGAGGGG-UCG--ACGGCUGCucCU- -5' |
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| 31882 | 5' | -62.1 | NC_006938.1 | + | 46589 | 1.1 | 0.00026 |
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Target: 5'- cCCGCGCUCCCCAGCUGCCGACGAGGAg -3' miRNA: 3'- -GGCGCGAGGGGUCGACGGCUGCUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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