Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31884 | 5' | -56.2 | NC_006938.1 | + | 47081 | 0.66 | 0.713707 |
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Target: 5'- uUGCcacccGUGUGGCGCaCGgugacgcgcgcccacUCGUCGGCGGu -3' miRNA: 3'- -ACGa----CGCACCGCG-GU---------------AGCAGUUGCUc -5' |
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| 31884 | 5' | -56.2 | NC_006938.1 | + | 58821 | 0.66 | 0.709467 |
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Target: 5'- aGCUcggaagGCGUGGUGCCGaaaUCAGCGGu -3' miRNA: 3'- aCGA------CGCACCGCGGUagcAGUUGCUc -5' |
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| 31884 | 5' | -56.2 | NC_006938.1 | + | 26066 | 0.66 | 0.709467 |
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Target: 5'- gGCUGCGUGGCaGCUg--GUCGcGCGGa -3' miRNA: 3'- aCGACGCACCG-CGGuagCAGU-UGCUc -5' |
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| 31884 | 5' | -56.2 | NC_006938.1 | + | 57275 | 0.66 | 0.688101 |
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Target: 5'- aGCUcCGUGGUGUCGagGUCAACu-- -3' miRNA: 3'- aCGAcGCACCGCGGUagCAGUUGcuc -5' |
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| 31884 | 5' | -56.2 | NC_006938.1 | + | 34446 | 0.69 | 0.48541 |
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Target: 5'- cUGCaGCGUGGUGCC-UCGguggcUCGGCGuGa -3' miRNA: 3'- -ACGaCGCACCGCGGuAGC-----AGUUGCuC- -5' |
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| 31884 | 5' | -56.2 | NC_006938.1 | + | 20668 | 0.7 | 0.436222 |
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Target: 5'- cUGCUGuUGUGGCGCCGcCGcCGugGuGa -3' miRNA: 3'- -ACGAC-GCACCGCGGUaGCaGUugCuC- -5' |
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| 31884 | 5' | -56.2 | NC_006938.1 | + | 53128 | 0.71 | 0.380307 |
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Target: 5'- gGCUGCcuccaccGUGGUGCCAgacugCG-CGGCGAGc -3' miRNA: 3'- aCGACG-------CACCGCGGUa----GCaGUUGCUC- -5' |
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| 31884 | 5' | -56.2 | NC_006938.1 | + | 45070 | 0.73 | 0.307765 |
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Target: 5'- aGUUGuCGUGGCGUCGUUGUC--CGAGu -3' miRNA: 3'- aCGAC-GCACCGCGGUAGCAGuuGCUC- -5' |
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| 31884 | 5' | -56.2 | NC_006938.1 | + | 18030 | 0.76 | 0.199509 |
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Target: 5'- aUGCgacaGCGcGGCGUCAagGUCGACGAGg -3' miRNA: 3'- -ACGa---CGCaCCGCGGUagCAGUUGCUC- -5' |
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| 31884 | 5' | -56.2 | NC_006938.1 | + | 48230 | 1.08 | 0.001055 |
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Target: 5'- gUGCUGCGUGGCGCCAUCGUCAACGAGu -3' miRNA: 3'- -ACGACGCACCGCGGUAGCAGUUGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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