Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31885 | 3' | -51.2 | NC_006938.1 | + | 58777 | 0.66 | 0.931415 |
Target: 5'- ----aGAUCagCGAGGCGGUUgGcCGAGg -3' miRNA: 3'- uuuugCUAGa-GCUCCGUUAGgC-GCUC- -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 37779 | 0.66 | 0.919559 |
Target: 5'- ---cCGAUCgcaucuUCGAGGU---CCGCGAGg -3' miRNA: 3'- uuuuGCUAG------AGCUCCGuuaGGCGCUC- -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 51676 | 0.66 | 0.919559 |
Target: 5'- -uGACGcccUCUCuGGGC--UCCGCGAGu -3' miRNA: 3'- uuUUGCu--AGAGcUCCGuuAGGCGCUC- -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 27331 | 0.66 | 0.913196 |
Target: 5'- cAGACGGg--CGAGacGCuGGUCCGCGAGa -3' miRNA: 3'- uUUUGCUagaGCUC--CG-UUAGGCGCUC- -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 36632 | 0.66 | 0.913196 |
Target: 5'- cGGAACGccgacGUCUCGcgacGGGCcaGGUCgGCGAGa -3' miRNA: 3'- -UUUUGC-----UAGAGC----UCCG--UUAGgCGCUC- -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 3837 | 0.67 | 0.892389 |
Target: 5'- -cGACGAg--CGucuuGGUGAUCUGCGAGg -3' miRNA: 3'- uuUUGCUagaGCu---CCGUUAGGCGCUC- -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 51634 | 0.67 | 0.869091 |
Target: 5'- -uGugGA---CGGGGCGGUCgGCGAGg -3' miRNA: 3'- uuUugCUagaGCUCCGUUAGgCGCUC- -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 29543 | 0.67 | 0.860799 |
Target: 5'- aGAGGCcGUCgaCGcGGguGUCCGCGAGg -3' miRNA: 3'- -UUUUGcUAGa-GCuCCguUAGGCGCUC- -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 6347 | 0.68 | 0.825208 |
Target: 5'- -uGGCGGUCUCGucGCuGUCCaCGAGc -3' miRNA: 3'- uuUUGCUAGAGCucCGuUAGGcGCUC- -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 51233 | 0.7 | 0.710862 |
Target: 5'- -cGACGAUCgCGGccggaauGGCAAUCCGCGc- -3' miRNA: 3'- uuUUGCUAGaGCU-------CCGUUAGGCGCuc -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 17243 | 0.72 | 0.611091 |
Target: 5'- -uGGCGAUCUCcAGGUccuGUCCgGCGAGg -3' miRNA: 3'- uuUUGCUAGAGcUCCGu--UAGG-CGCUC- -5' |
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31885 | 3' | -51.2 | NC_006938.1 | + | 48940 | 1.06 | 0.004532 |
Target: 5'- gAAAACGAUCUCGAGGCAAUCCGCGAGc -3' miRNA: 3'- -UUUUGCUAGAGCUCCGUUAGGCGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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