Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31886 | 5' | -47.9 | NC_006938.1 | + | 36604 | 0.66 | 0.98597 |
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Target: 5'- -gGUCGgCGAGAGGGA---UCUCAUCc -3' miRNA: 3'- agUAGCgGUUCUUCUUcagAGAGUAG- -5' |
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| 31886 | 5' | -47.9 | NC_006938.1 | + | 17774 | 0.68 | 0.961424 |
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Target: 5'- cCGUCGCCGGGGacuggcucggagagcAGAAGUCcaagaccCUCAUg -3' miRNA: 3'- aGUAGCGGUUCU---------------UCUUCAGa------GAGUAg -5' |
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| 31886 | 5' | -47.9 | NC_006938.1 | + | 35194 | 0.68 | 0.95112 |
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Target: 5'- gUCAUCGCCAugcugcacGAGGUCaUCUCGg- -3' miRNA: 3'- -AGUAGCGGUucuu----CUUCAG-AGAGUag -5' |
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| 31886 | 5' | -47.9 | NC_006938.1 | + | 56141 | 0.68 | 0.94633 |
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Target: 5'- -uGUCGaCCuGGAAGGAGgcgCUgUCGUCa -3' miRNA: 3'- agUAGC-GGuUCUUCUUCa--GAgAGUAG- -5' |
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| 31886 | 5' | -47.9 | NC_006938.1 | + | 5577 | 0.69 | 0.941241 |
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Target: 5'- ----gGCCGAGAAGGAGgucgccgCUCUCGa- -3' miRNA: 3'- aguagCGGUUCUUCUUCa------GAGAGUag -5' |
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| 31886 | 5' | -47.9 | NC_006938.1 | + | 35679 | 0.69 | 0.93585 |
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Target: 5'- cCGUCGCCAGGGAGGAGcccaagUUCcUGUCc -3' miRNA: 3'- aGUAGCGGUUCUUCUUCa-----GAGaGUAG- -5' |
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| 31886 | 5' | -47.9 | NC_006938.1 | + | 62559 | 0.69 | 0.93585 |
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Target: 5'- gCGUCggcgGCCGAGGAGuuccGUCUCaUCGUCc -3' miRNA: 3'- aGUAG----CGGUUCUUCuu--CAGAG-AGUAG- -5' |
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| 31886 | 5' | -47.9 | NC_006938.1 | + | 47312 | 0.71 | 0.873719 |
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Target: 5'- -gAUCGCCGAGGAaGAGaCUCUCGg- -3' miRNA: 3'- agUAGCGGUUCUUcUUCaGAGAGUag -5' |
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| 31886 | 5' | -47.9 | NC_006938.1 | + | 7440 | 0.73 | 0.778193 |
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Target: 5'- uUCGUCgaacugaGCCAGGAc--GGUCUCUCGUCc -3' miRNA: 3'- -AGUAG-------CGGUUCUucuUCAGAGAGUAG- -5' |
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| 31886 | 5' | -47.9 | NC_006938.1 | + | 49263 | 1.1 | 0.005231 |
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Target: 5'- cUCAUCGCCAAGAAGAAGUCUCUCAUCc -3' miRNA: 3'- -AGUAGCGGUUCUUCUUCAGAGAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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