Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31887 | 3' | -52.8 | NC_006938.1 | + | 9587 | 0.68 | 0.739272 |
Target: 5'- gGGCUgcucgccgCAGGGAuUGGUGGCGGCGa--- -3' miRNA: 3'- aCCGA--------GUUCUU-GCCACCGUCGUaugu -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 11066 | 0.7 | 0.639627 |
Target: 5'- aUGGCUCcccAGAcccggccgAUGuGUGGCAGCAagUGCAg -3' miRNA: 3'- -ACCGAGu--UCU--------UGC-CACCGUCGU--AUGU- -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 51110 | 0.7 | 0.639627 |
Target: 5'- cGGCg-GAGGcccCGGUGGCAGCGUcgaACGc -3' miRNA: 3'- aCCGagUUCUu--GCCACCGUCGUA---UGU- -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 11263 | 0.7 | 0.605872 |
Target: 5'- cGGCgUCGAGGA-GGaUGGCGGCGgugGCGa -3' miRNA: 3'- aCCG-AGUUCUUgCC-ACCGUCGUa--UGU- -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 49552 | 1.09 | 0.0017 |
Target: 5'- cUGGCUCAAGAACGGUGGCAGCAUACAg -3' miRNA: 3'- -ACCGAGUUCUUGCCACCGUCGUAUGU- -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 56758 | 0.74 | 0.38714 |
Target: 5'- cUGGUUCGucgcccAGucagGCGGUGGCAGCAUGa- -3' miRNA: 3'- -ACCGAGU------UCu---UGCCACCGUCGUAUgu -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 63509 | 0.7 | 0.627242 |
Target: 5'- cUGGCgcacguUCAAGGACGGaccgcacUGGCAGC-UGCc -3' miRNA: 3'- -ACCG------AGUUCUUGCC-------ACCGUCGuAUGu -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 52185 | 0.68 | 0.706637 |
Target: 5'- aGGCgguGGAgGCGGUGGUGGCAUcuucgGCGg -3' miRNA: 3'- aCCGaguUCU-UGCCACCGUCGUA-----UGU- -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 52302 | 0.68 | 0.72849 |
Target: 5'- cGGCUCGAuccucaucGGCGGccagGGCGGCAUcgACAa -3' miRNA: 3'- aCCGAGUUc-------UUGCCa---CCGUCGUA--UGU- -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 44257 | 0.67 | 0.760483 |
Target: 5'- aGGCccucuUCAAGGGaGGUGGCGGUggGCu -3' miRNA: 3'- aCCG-----AGUUCUUgCCACCGUCGuaUGu -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 59598 | 0.67 | 0.760483 |
Target: 5'- cGGCaagcaccuUCAAcGGCGGUGGCAuCGUGCu -3' miRNA: 3'- aCCG--------AGUUcUUGCCACCGUcGUAUGu -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 15468 | 0.67 | 0.770889 |
Target: 5'- cUGGCcucgacCAGGAGCGGgcugacgcgGGCGGCAaggACGu -3' miRNA: 3'- -ACCGa-----GUUCUUGCCa--------CCGUCGUa--UGU- -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 28951 | 0.66 | 0.820433 |
Target: 5'- aGGCUCGAGGcCGcUGGCcugGGCGggACAg -3' miRNA: 3'- aCCGAGUUCUuGCcACCG---UCGUa-UGU- -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 32844 | 0.66 | 0.836169 |
Target: 5'- gUGGCUCGAGGGCagcugggacaucguGGccGGUGGCAUGu- -3' miRNA: 3'- -ACCGAGUUCUUG--------------CCa-CCGUCGUAUgu -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 45669 | 0.66 | 0.83888 |
Target: 5'- aGGC-CGAGAACGGcUGG-AGCGgACu -3' miRNA: 3'- aCCGaGUUCUUGCC-ACCgUCGUaUGu -5' |
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31887 | 3' | -52.8 | NC_006938.1 | + | 37918 | 0.66 | 0.847767 |
Target: 5'- gGGCggCGAGcgGGCGGUGGCcagGGCGguggagGCGu -3' miRNA: 3'- aCCGa-GUUC--UUGCCACCG---UCGUa-----UGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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