Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31887 | 5' | -59 | NC_006938.1 | + | 34360 | 0.69 | 0.345168 |
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Target: 5'- aGACGCccugGCccUGCGGCuCGUugaccuccgccUCGCCGAGGa -3' miRNA: 3'- -UUGCGa---CG--AUGUCG-GCG-----------AGCGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 52734 | 0.7 | 0.33715 |
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Target: 5'- -uCGCUGUUGC-GCCGCUCGaaacccgaCGAGa -3' miRNA: 3'- uuGCGACGAUGuCGGCGAGCg-------GCUCc -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 19793 | 0.7 | 0.336355 |
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Target: 5'- cGCGCUGCUAagugGGaCCgugacuaccucaaGCUCGCCGAGu -3' miRNA: 3'- uUGCGACGAUg---UC-GG-------------CGAGCGGCUCc -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 26066 | 0.7 | 0.306443 |
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Target: 5'- --gGCUGCgugGCAGCUGgUCGCgCgGAGGa -3' miRNA: 3'- uugCGACGa--UGUCGGCgAGCG-G-CUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 46012 | 0.71 | 0.28485 |
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Target: 5'- -gUGCUGCUGaaucaGGCCGCguggGCCGAGa -3' miRNA: 3'- uuGCGACGAUg----UCGGCGag--CGGCUCc -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 17137 | 0.72 | 0.23917 |
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Target: 5'- --aGCUGCUcgaugaccuCGGCCGCUCGCuuguacuggauCGAGGa -3' miRNA: 3'- uugCGACGAu--------GUCGGCGAGCG-----------GCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 7807 | 0.77 | 0.101909 |
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Target: 5'- -cUGCUGCUGCAGcCCGUUCaGCCGGGu -3' miRNA: 3'- uuGCGACGAUGUC-GGCGAG-CGGCUCc -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 49588 | 1.08 | 0.000502 |
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Target: 5'- gAACGCUGCUACAGCCGCUCGCCGAGGg -3' miRNA: 3'- -UUGCGACGAUGUCGGCGAGCGGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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