Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31889 | 3' | -56.6 | NC_006938.1 | + | 23585 | 0.68 | 0.540066 |
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Target: 5'- cUCGCGGCUGGcuUCGaGCUCGGCGa -3' miRNA: 3'- uAGUGCCGGCUccAGCaCGAGUUGCg -5' |
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| 31889 | 3' | -56.6 | NC_006938.1 | + | 32928 | 0.69 | 0.468545 |
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Target: 5'- cAUCAU--CCGAGGUCGUggaaGCUCGACcGCu -3' miRNA: 3'- -UAGUGccGGCUCCAGCA----CGAGUUG-CG- -5' |
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| 31889 | 3' | -56.6 | NC_006938.1 | + | 60886 | 0.7 | 0.443224 |
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Target: 5'- cUCuuCGG-CGAGGUCGUGUUCcucuccaacuucgagGACGCg -3' miRNA: 3'- uAGu-GCCgGCUCCAGCACGAG---------------UUGCG- -5' |
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| 31889 | 3' | -56.6 | NC_006938.1 | + | 30720 | 0.71 | 0.393218 |
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Target: 5'- gGUCGCGGCUcGGGcgcguUCGUGCUCccGACGa -3' miRNA: 3'- -UAGUGCCGGcUCC-----AGCACGAG--UUGCg -5' |
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| 31889 | 3' | -56.6 | NC_006938.1 | + | 10192 | 0.72 | 0.326204 |
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Target: 5'- gGUCACGGCCGAGGagcaCGUGgacguuCUCAcaguCGCc -3' miRNA: 3'- -UAGUGCCGGCUCCa---GCAC------GAGUu---GCG- -5' |
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| 31889 | 3' | -56.6 | NC_006938.1 | + | 40139 | 0.75 | 0.230143 |
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Target: 5'- uGUCGCGGCCGAGGUgGacaUGacaGACGCu -3' miRNA: 3'- -UAGUGCCGGCUCCAgC---ACgagUUGCG- -5' |
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| 31889 | 3' | -56.6 | NC_006938.1 | + | 8297 | 0.76 | 0.181854 |
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Target: 5'- uGUUGCGGCCGcauauaagcuGGUCG-GCUCGACGUg -3' miRNA: 3'- -UAGUGCCGGCu---------CCAGCaCGAGUUGCG- -5' |
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| 31889 | 3' | -56.6 | NC_006938.1 | + | 50885 | 1 | 0.004005 |
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Target: 5'- gAUCACGGCCGAGG-CGUGCUCAACGCc -3' miRNA: 3'- -UAGUGCCGGCUCCaGCACGAGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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