miRNA: 3'- gUUC-CUGgAguGGAGCuGCCUGGUCc -5'
miRNA: 3'- guucCUGGAG---UGGAGCU-GCCuGGUCC- -5'
miRNA: 3'- guuCCUGG---------AGUGGaGCugCCUGGUCC- -5'
miRNA: 3'- guUCCU-GGAGUGGA-------GCUGCCuGGUCC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31896 | 5' | -56.2 | NC_006938.1 | + | 20454 | 0.66 | 0.738257 |
|
Target: 5'- gGAGcGGCgUguCCUCG-CGGACCGGc -3' miRNA: 3'- gUUC-CUGgAguGGAGCuGCCUGGUCc -5' |
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| 31896 | 5' | -56.2 | NC_006938.1 | + | 45736 | 0.66 | 0.748416 |
|
Target: 5'- ---cGGCCUCccgGCCUCGAuCGG-UCAGGu -3' miRNA: 3'- guucCUGGAG---UGGAGCU-GCCuGGUCC- -5' |
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| 31896 | 5' | -56.2 | NC_006938.1 | + | 48285 | 0.66 | 0.758464 |
|
Target: 5'- --uGGACCugcguguugUCGCC-CGugGGAUUGGGc -3' miRNA: 3'- guuCCUGG---------AGUGGaGCugCCUGGUCC- -5' |
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| 31896 | 5' | -56.2 | NC_006938.1 | + | 38734 | 0.75 | 0.24855 |
|
Target: 5'- -cGGGAuCCUCACCUgccagagCGGCGG-CCAGGu -3' miRNA: 3'- guUCCU-GGAGUGGA-------GCUGCCuGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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