Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
319 | 5' | -55.9 | AC_000010.1 | + | 10542 | 0.66 | 0.536708 |
Target: 5'- gUACUGgCACUCCCgucucgACCCAAG-CCuGCa -3' miRNA: 3'- -GUGGUgGUGAGGG------UGGGUUCaGGuUG- -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 26107 | 0.66 | 0.536708 |
Target: 5'- aGCUACCAUcUCCGCuCCGGGUcggggggcCCAGCa -3' miRNA: 3'- gUGGUGGUGaGGGUG-GGUUCA--------GGUUG- -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 19592 | 0.66 | 0.536708 |
Target: 5'- aGCCAgCACUCCCAUguugccaguaCUguuauaguaauGAGUCCGAUg -3' miRNA: 3'- gUGGUgGUGAGGGUG----------GG-----------UUCAGGUUG- -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 10726 | 0.66 | 0.514795 |
Target: 5'- gCGCgaGCCACUCCCagGCCCGAGaacugagcguUCUAAa -3' miRNA: 3'- -GUGg-UGGUGAGGG--UGGGUUC----------AGGUUg -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 24789 | 0.67 | 0.493255 |
Target: 5'- cUugCGCCGCUCU--UCCAAGUCUAGu -3' miRNA: 3'- -GugGUGGUGAGGguGGGUUCAGGUUg -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 23780 | 0.67 | 0.472138 |
Target: 5'- cCACCACCACcUCUACCCucgaggauGAGgaggUCGACg -3' miRNA: 3'- -GUGGUGGUGaGGGUGGG--------UUCa---GGUUG- -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 27995 | 0.67 | 0.461752 |
Target: 5'- aUACUACCACUCCCagaACCgGAGgUgAGCu -3' miRNA: 3'- -GUGGUGGUGAGGG---UGGgUUCaGgUUG- -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 27045 | 0.67 | 0.460719 |
Target: 5'- cCACCACCguacuACUUCCucgagacGCCCAGG-CCGAa -3' miRNA: 3'- -GUGGUGG-----UGAGGG-------UGGGUUCaGGUUg -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 10341 | 0.68 | 0.431341 |
Target: 5'- cCACCACCGcCUCCgguauCACCUggauGUCCAGg -3' miRNA: 3'- -GUGGUGGU-GAGG-----GUGGGuu--CAGGUUg -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 25373 | 0.68 | 0.402134 |
Target: 5'- uGCUACCugUUCCGCCUGg--CCAACu -3' miRNA: 3'- gUGGUGGugAGGGUGGGUucaGGUUG- -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 2264 | 0.69 | 0.383374 |
Target: 5'- uGCC-CCugUCCCGCCCAcuGGUUguAg -3' miRNA: 3'- gUGGuGGugAGGGUGGGU--UCAGguUg -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 581 | 0.69 | 0.374215 |
Target: 5'- aCACCugCGCUUCCuguCCCAGGagauagucUCCAc- -3' miRNA: 3'- -GUGGugGUGAGGGu--GGGUUC--------AGGUug -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 11094 | 0.69 | 0.365205 |
Target: 5'- gACgCGCCAgUCCCucgcccucuUCCAAGUCCAGa -3' miRNA: 3'- gUG-GUGGUgAGGGu--------GGGUUCAGGUUg -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 8458 | 0.69 | 0.347639 |
Target: 5'- aCACCACCGCcUCCGCCCAaaAGgaaaAACa -3' miRNA: 3'- -GUGGUGGUGaGGGUGGGU--UCagg-UUG- -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 13982 | 0.7 | 0.330685 |
Target: 5'- aCACCACCGCUCCU-CCUAgcacGGUUCGc- -3' miRNA: 3'- -GUGGUGGUGAGGGuGGGU----UCAGGUug -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 10016 | 0.72 | 0.229135 |
Target: 5'- aGCCACC---CUCACCCAGGUgCAGCa -3' miRNA: 3'- gUGGUGGugaGGGUGGGUUCAgGUUG- -5' |
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319 | 5' | -55.9 | AC_000010.1 | + | 13825 | 1.1 | 0.000378 |
Target: 5'- cCACCACCACUCCCACCCAAGUCCAACa -3' miRNA: 3'- -GUGGUGGUGAGGGUGGGUUCAGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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