Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31900 | 5' | -54.4 | NC_006938.1 | + | 42970 | 0.66 | 0.795044 |
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Target: 5'- cUACAcUGUGUucaauggcacgacgaUCGACGACCUGgCGaCg -3' miRNA: 3'- -AUGUaGCACA---------------AGCUGCUGGACgGC-Ga -5' |
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| 31900 | 5' | -54.4 | NC_006938.1 | + | 11561 | 0.66 | 0.761058 |
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Target: 5'- cGCGUCGUGUUCGucggGCGGCagGUgGCg -3' miRNA: 3'- aUGUAGCACAAGC----UGCUGgaCGgCGa -5' |
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| 31900 | 5' | -54.4 | NC_006938.1 | + | 29461 | 0.66 | 0.761058 |
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Target: 5'- gGCAUgcUGUUCGACGAgaccaacucUCUGCUGCc -3' miRNA: 3'- aUGUAgcACAAGCUGCU---------GGACGGCGa -5' |
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| 31900 | 5' | -54.4 | NC_006938.1 | + | 34414 | 0.67 | 0.750751 |
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Target: 5'- gACAUCGUGc-CGGagccaGACCaGCCGCc -3' miRNA: 3'- aUGUAGCACaaGCUg----CUGGaCGGCGa -5' |
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| 31900 | 5' | -54.4 | NC_006938.1 | + | 46270 | 0.67 | 0.740323 |
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Target: 5'- gGCGUCGUG---GAUGGCCUuggcaaugGCCGCg -3' miRNA: 3'- aUGUAGCACaagCUGCUGGA--------CGGCGa -5' |
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| 31900 | 5' | -54.4 | NC_006938.1 | + | 198 | 0.68 | 0.686773 |
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Target: 5'- cACcgCGUGggCGAcCGGCUcGCCGCc -3' miRNA: 3'- aUGuaGCACaaGCU-GCUGGaCGGCGa -5' |
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| 31900 | 5' | -54.4 | NC_006938.1 | + | 14356 | 0.7 | 0.577022 |
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Target: 5'- -uCGUUGUGgaUGGCGGCCUGCUGg- -3' miRNA: 3'- auGUAGCACaaGCUGCUGGACGGCga -5' |
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| 31900 | 5' | -54.4 | NC_006938.1 | + | 33016 | 0.7 | 0.577022 |
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Target: 5'- gACAUCGcccaUGUUCGcCGuCUUGCCGUUg -3' miRNA: 3'- aUGUAGC----ACAAGCuGCuGGACGGCGA- -5' |
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| 31900 | 5' | -54.4 | NC_006938.1 | + | 54547 | 0.7 | 0.523261 |
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Target: 5'- cGCAUgGUGg-CGGCGGCCUGCUugGCg -3' miRNA: 3'- aUGUAgCACaaGCUGCUGGACGG--CGa -5' |
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| 31900 | 5' | -54.4 | NC_006938.1 | + | 55933 | 1.06 | 0.002091 |
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Target: 5'- cUACAUCGUGUUCGACGACCUGCCGCUg -3' miRNA: 3'- -AUGUAGCACAAGCUGCUGGACGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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