Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31901 | 3' | -56.6 | NC_006938.1 | + | 30068 | 0.66 | 0.712599 |
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Target: 5'- -gGUGgGGAUGGgGacccgUCggCGGCAUGg -3' miRNA: 3'- agCACgCCUGCCgCa----AGaaGCCGUGC- -5' |
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| 31901 | 3' | -56.6 | NC_006938.1 | + | 15655 | 0.66 | 0.712599 |
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Target: 5'- ---cGCGGuCGGCGUcgaggUCggUCGGUACu -3' miRNA: 3'- agcaCGCCuGCCGCA-----AGa-AGCCGUGc -5' |
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| 31901 | 3' | -56.6 | NC_006938.1 | + | 10112 | 0.66 | 0.702155 |
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Target: 5'- --aUGcCGGACGGUGUUCgcaCGGaGCGg -3' miRNA: 3'- agcAC-GCCUGCCGCAAGaa-GCCgUGC- -5' |
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| 31901 | 3' | -56.6 | NC_006938.1 | + | 17480 | 0.67 | 0.659836 |
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Target: 5'- cCGUGCGGACgGGCGgucacaUCgcUGGCuAUGg -3' miRNA: 3'- aGCACGCCUG-CCGCa-----AGaaGCCG-UGC- -5' |
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| 31901 | 3' | -56.6 | NC_006938.1 | + | 62701 | 0.69 | 0.532792 |
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Target: 5'- cUCG-GCaGGGCGGCGUcuggcgcaUCUUCGuGgACGg -3' miRNA: 3'- -AGCaCG-CCUGCCGCA--------AGAAGC-CgUGC- -5' |
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| 31901 | 3' | -56.6 | NC_006938.1 | + | 52078 | 0.71 | 0.406463 |
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Target: 5'- uUCGUGCGGAcCGGCGaguucgaCUUCGaGCAg- -3' miRNA: 3'- -AGCACGCCU-GCCGCaa-----GAAGC-CGUgc -5' |
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| 31901 | 3' | -56.6 | NC_006938.1 | + | 33262 | 0.77 | 0.191032 |
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Target: 5'- uUCGUGCGGACGGgGUUCUUCa----- -3' miRNA: 3'- -AGCACGCCUGCCgCAAGAAGccgugc -5' |
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| 31901 | 3' | -56.6 | NC_006938.1 | + | 56288 | 1.1 | 0.000901 |
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Target: 5'- uUCGUGCGGACGGCGUUCUUCGGCACGg -3' miRNA: 3'- -AGCACGCCUGCCGCAAGAAGCCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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