Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31904 | 5' | -50.8 | NC_006938.1 | + | 15966 | 0.66 | 0.914938 |
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Target: 5'- gAUCGCCgacgcgGUCGAGAUccucuCCCAGAu -3' miRNA: 3'- -UAGCGGa-----UAGCUCUAcacu-GGGUUUu -5' |
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| 31904 | 5' | -50.8 | NC_006938.1 | + | 1473 | 0.66 | 0.908243 |
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Target: 5'- -cUGCCUccCGAGGgcgagGUGGCCCGGu- -3' miRNA: 3'- uaGCGGAuaGCUCUa----CACUGGGUUuu -5' |
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| 31904 | 5' | -50.8 | NC_006938.1 | + | 37786 | 0.67 | 0.853338 |
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Target: 5'- --gGCCUcgcUCGGGGagGUGGCCCAGAc -3' miRNA: 3'- uagCGGAu--AGCUCUa-CACUGGGUUUu -5' |
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| 31904 | 5' | -50.8 | NC_006938.1 | + | 62535 | 0.69 | 0.796413 |
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Target: 5'- -gCGCCUcugUGAGGUGUauGACCCGAu- -3' miRNA: 3'- uaGCGGAua-GCUCUACA--CUGGGUUuu -5' |
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| 31904 | 5' | -50.8 | NC_006938.1 | + | 49901 | 0.71 | 0.688006 |
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Target: 5'- gGUCGaaggGUCGAGGUGaacgGACCCGAGAu -3' miRNA: 3'- -UAGCgga-UAGCUCUACa---CUGGGUUUU- -5' |
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| 31904 | 5' | -50.8 | NC_006938.1 | + | 58232 | 1.04 | 0.005573 |
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Target: 5'- gAUCGCCUAUCGAGAUGUGACCCAAAAc -3' miRNA: 3'- -UAGCGGAUAGCUCUACACUGGGUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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