miRNA: 3'- -ACCGUU--CAa--GgGUGCCUUGCGCUGgu -5'
miRNA: 3'- -ACCG---UUCAAGGGUgcCUugCGCUGGU- -5'
miRNA: 3'- -ACCGUUCaaGGgUGCCUUGCGCuGGU- -5'
miRNA: 3'- aCCGUUCAA-GGGU-----GCCU----UGCGCUGGu -5'
miRNA: 3'- -ACCGUUCAAGGGUGCCUUGCGCUGGU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31908 | 5' | -55.5 | NC_006938.1 | + | 12392 | 0.72 | 0.384854 |
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Target: 5'- aUGGCAGccGUcagCgCGCGGAACGCGACg- -3' miRNA: 3'- -ACCGUU--CAa--GgGUGCCUUGCGCUGgu -5' |
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| 31908 | 5' | -55.5 | NC_006938.1 | + | 62993 | 0.73 | 0.334201 |
|
Target: 5'- cUGGCcuuGGGUUCCCAgcGAugGUGACCAu -3' miRNA: 3'- -ACCG---UUCAAGGGUgcCUugCGCUGGU- -5' |
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| 31908 | 5' | -55.5 | NC_006938.1 | + | 12278 | 0.73 | 0.334201 |
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Target: 5'- gUGGCGAG--CUgGCGGAACGCG-CCAc -3' miRNA: 3'- -ACCGUUCaaGGgUGCCUUGCGCuGGU- -5' |
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| 31908 | 5' | -55.5 | NC_006938.1 | + | 41893 | 0.74 | 0.310767 |
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Target: 5'- gGGCAGGUUgCCCGacagcUGGAccgcGCGCGGCCc -3' miRNA: 3'- aCCGUUCAA-GGGU-----GCCU----UGCGCUGGu -5' |
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| 31908 | 5' | -55.5 | NC_006938.1 | + | 62230 | 1.09 | 0.001152 |
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Target: 5'- uUGGCAAGUUCCCACGGAACGCGACCAa -3' miRNA: 3'- -ACCGUUCAAGGGUGCCUUGCGCUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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