miRNA: 3'- ucCCGCCGCCGcucgGCCAGCGGgUG- -5'
miRNA: 3'- ucCCGCCGCCG--CUCGGCCaGC-GgGUG- -5'
miRNA: 3'- uCCCGCCgccgCUCGGCCAG------------CGGGUg -5'
miRNA: 3'- ucCCGCCGCCg-CU-CGGCCAg-------------CGGGUg -5'
miRNA: 3'- -UCCcGCCGCCGCUCggCCaGCGGGUg -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31911 | 5' | -67 | NC_006938.1 | + | 29990 | 0.66 | 0.226323 |
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Target: 5'- -cGGUGGCGGCa---CGGUUGCCgACg -3' miRNA: 3'- ucCCGCCGCCGcucgGCCAGCGGgUG- -5' |
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| 31911 | 5' | -67 | NC_006938.1 | + | 54397 | 0.66 | 0.237478 |
|
Target: 5'- -cGGCGGUGGCcaGAGCUGG-CGaCgCACu -3' miRNA: 3'- ucCCGCCGCCG--CUCGGCCaGC-GgGUG- -5' |
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| 31911 | 5' | -67 | NC_006938.1 | + | 28970 | 0.66 | 0.237478 |
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Target: 5'- uGGGCGGgacaGAGCCGGUCcggauggacacgGCCCGa -3' miRNA: 3'- uCCCGCCgccgCUCGGCCAG------------CGGGUg -5' |
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| 31911 | 5' | -67 | NC_006938.1 | + | 5714 | 0.66 | 0.245559 |
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Target: 5'- -uGGCGGCGGgaGAgGCCGGUggcuucuggggaaaGCCCAg -3' miRNA: 3'- ucCCGCCGCCg-CU-CGGCCAg-------------CGGGUg -5' |
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| 31911 | 5' | -67 | NC_006938.1 | + | 27715 | 0.66 | 0.249091 |
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Target: 5'- aAGGuCGGUGGCGAGaaGGgUGUCCAa -3' miRNA: 3'- -UCCcGCCGCCGCUCggCCaGCGGGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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