Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31912 | 5' | -59.6 | NC_006938.1 | + | 20898 | 0.68 | 0.434462 |
Target: 5'- aGGUGGUGGAGCcgccauAGGCUGUUCccaGGGa -3' miRNA: 3'- -CCGCCACUUCG------UCCGGCGAGcg-CUCg -5' |
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31912 | 5' | -59.6 | NC_006938.1 | + | 20350 | 0.69 | 0.37259 |
Target: 5'- aGCaGGUGGacAGCuGGCCGgUCcGCGAGg -3' miRNA: 3'- cCG-CCACU--UCGuCCGGCgAG-CGCUCg -5' |
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31912 | 5' | -59.6 | NC_006938.1 | + | 15469 | 0.67 | 0.462741 |
Target: 5'- uGGCcucgaccaGGAGCGGGCUGa-CGCGGGCg -3' miRNA: 3'- -CCGcca-----CUUCGUCCGGCgaGCGCUCG- -5' |
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31912 | 5' | -59.6 | NC_006938.1 | + | 11871 | 0.67 | 0.443778 |
Target: 5'- aGUGG-GGAGCcGGuuGaCUCGCgGAGCc -3' miRNA: 3'- cCGCCaCUUCGuCCggC-GAGCG-CUCG- -5' |
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31912 | 5' | -59.6 | NC_006938.1 | + | 8145 | 0.73 | 0.208598 |
Target: 5'- gGGCGa-GAAGCccAGGCCGCcugCGgCGAGCg -3' miRNA: 3'- -CCGCcaCUUCG--UCCGGCGa--GC-GCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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