Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31920 | 3' | -37.7 | NC_006940.1 | + | 29715 | 0.68 | 1 |
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Target: 5'- cGCggcgAAGAC---GUC-GAAGUGUUUGCa -3' miRNA: 3'- -CGa---UUCUGauuUAGuCUUUAUAAGCG- -5' |
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| 31920 | 3' | -37.7 | NC_006940.1 | + | 34886 | 0.66 | 1 |
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Target: 5'- cGgUGAGACUGuuguUCGGGGAauccgccugcgaGUUCGCa -3' miRNA: 3'- -CgAUUCUGAUuu--AGUCUUUa-----------UAAGCG- -5' |
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| 31920 | 3' | -37.7 | NC_006940.1 | + | 11843 | 0.66 | 1 |
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Target: 5'- gGCUucGGCacaggUAAAUCAGAGGUcaugUGCa -3' miRNA: 3'- -CGAuuCUG-----AUUUAGUCUUUAuaa-GCG- -5' |
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| 31920 | 3' | -37.7 | NC_006940.1 | + | 20801 | 0.69 | 0.999999 |
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Target: 5'- cGCUucGGCccgUGAGUCGGuuAUGUcCGCu -3' miRNA: 3'- -CGAuuCUG---AUUUAGUCuuUAUAaGCG- -5' |
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| 31920 | 3' | -37.7 | NC_006940.1 | + | 5774 | 0.72 | 0.999925 |
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Target: 5'- gGCUcAAGGC-AAAUCAGGAAg---CGCu -3' miRNA: 3'- -CGA-UUCUGaUUUAGUCUUUauaaGCG- -5' |
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| 31920 | 3' | -37.7 | NC_006940.1 | + | 2595 | 0.73 | 0.999741 |
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Target: 5'- aGCccGGACUGGuuAUCAGGGAaGUUUGCc -3' miRNA: 3'- -CGauUCUGAUU--UAGUCUUUaUAAGCG- -5' |
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| 31920 | 3' | -37.7 | NC_006940.1 | + | 2700 | 1.19 | 0.020612 |
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Target: 5'- gGCUAAGACUAAAUCAGAAAUAUUCGCc -3' miRNA: 3'- -CGAUUCUGAUUUAGUCUUUAUAAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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