Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31922 | 3' | -50.4 | NC_006940.1 | + | 10185 | 0.67 | 0.818083 |
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Target: 5'- -gGugGCcGCGGCUGUU-UCCGGAgCAu -3' miRNA: 3'- caUugCGaCGUUGGCAAuAGGCCU-GU- -5' |
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| 31922 | 3' | -50.4 | NC_006940.1 | + | 26481 | 0.68 | 0.77705 |
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Target: 5'- -gAACGCgauUGCucAACCgGUUGUCCGcGGCAa -3' miRNA: 3'- caUUGCG---ACG--UUGG-CAAUAGGC-CUGU- -5' |
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| 31922 | 3' | -50.4 | NC_006940.1 | + | 20384 | 0.69 | 0.718664 |
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Target: 5'- -aGGCGCUGUAGgCGUUGUCCaccacaccccgaaaGGugGa -3' miRNA: 3'- caUUGCGACGUUgGCAAUAGG--------------CCugU- -5' |
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| 31922 | 3' | -50.4 | NC_006940.1 | + | 4771 | 0.73 | 0.503452 |
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Target: 5'- cUGACGCUGcCAGaaGUUAUCCaGACGg -3' miRNA: 3'- cAUUGCGAC-GUUggCAAUAGGcCUGU- -5' |
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| 31922 | 3' | -50.4 | NC_006940.1 | + | 15651 | 0.77 | 0.286165 |
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Target: 5'- gGUAACGCUGCuGCCGUaggcUCCGGGg- -3' miRNA: 3'- -CAUUGCGACGuUGGCAau--AGGCCUgu -5' |
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| 31922 | 3' | -50.4 | NC_006940.1 | + | 7776 | 1.1 | 0.001838 |
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Target: 5'- cGUAACGCUGCAACCGUUAUCCGGACAg -3' miRNA: 3'- -CAUUGCGACGUUGGCAAUAGGCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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