Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31922 | 5' | -50.1 | NC_006940.1 | + | 1462 | 0.66 | 0.917553 |
Target: 5'- cCGUAUgCgCAACGucugGAGUACGGUuggucucAACAGGc -3' miRNA: 3'- -GCAUA-G-GUUGC----UUCGUGCCG-------UUGUCC- -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 7431 | 0.66 | 0.897519 |
Target: 5'- gCGUGguUCCAggACGAAGCACuuGGCcccguaAGGg -3' miRNA: 3'- -GCAU--AGGU--UGCUUCGUG--CCGuug---UCC- -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 15578 | 0.66 | 0.89003 |
Target: 5'- aCGgagCCc-CGGAGCcuACGGCAGCAGc -3' miRNA: 3'- -GCauaGGuuGCUUCG--UGCCGUUGUCc -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 35534 | 0.67 | 0.857243 |
Target: 5'- cCGaUGUUCGAUGuaacAAGCccaACGGCAAUAGGc -3' miRNA: 3'- -GC-AUAGGUUGC----UUCG---UGCCGUUGUCC- -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 714 | 0.67 | 0.857243 |
Target: 5'- aCGcUAUUgAACuGAGCGCGGCcaguCAGGa -3' miRNA: 3'- -GC-AUAGgUUGcUUCGUGCCGuu--GUCC- -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 28328 | 0.68 | 0.839257 |
Target: 5'- cCGUucgacUCGACGAacacAGUaucaACGGCAACAGGc -3' miRNA: 3'- -GCAua---GGUUGCU----UCG----UGCCGUUGUCC- -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 14448 | 0.7 | 0.72566 |
Target: 5'- gCGcUAUUCGACGGuauGCuguGGCAACAGGc -3' miRNA: 3'- -GC-AUAGGUUGCUu--CGug-CCGUUGUCC- -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 19698 | 0.71 | 0.634209 |
Target: 5'- gCGUGcUCCGGCGuGGCG-GGCAuuuGCAGGc -3' miRNA: 3'- -GCAU-AGGUUGCuUCGUgCCGU---UGUCC- -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 15603 | 0.72 | 0.611082 |
Target: 5'- -aUGUUCAugGgcGCgACGGCAACGGa -3' miRNA: 3'- gcAUAGGUugCuuCG-UGCCGUUGUCc -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 1548 | 0.72 | 0.576569 |
Target: 5'- --aAUCCGGC-AA-CACGGCAACAGGu -3' miRNA: 3'- gcaUAGGUUGcUUcGUGCCGUUGUCC- -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 36883 | 0.74 | 0.498204 |
Target: 5'- cCGUGUCCGaacgGCGGAGCAgauGCGACAGa -3' miRNA: 3'- -GCAUAGGU----UGCUUCGUgc-CGUUGUCc -5' |
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31922 | 5' | -50.1 | NC_006940.1 | + | 7739 | 1.12 | 0.001697 |
Target: 5'- aCGUAUCCAACGAAGCACGGCAACAGGu -3' miRNA: 3'- -GCAUAGGUUGCUUCGUGCCGUUGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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