Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31924 | 3' | -47.8 | NC_006940.1 | + | 26505 | 0.66 | 0.964696 |
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Target: 5'- cCGCGGCaaucgu-GCgGACaACAAGCCc -3' miRNA: 3'- -GUGCCGgauauauUGaCUG-UGUUCGG- -5' |
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| 31924 | 3' | -47.8 | NC_006940.1 | + | 19356 | 0.66 | 0.961545 |
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Target: 5'- gUACGGCCUGU-----UGGCACAcuuccuuaaaGGUCg -3' miRNA: 3'- -GUGCCGGAUAuauugACUGUGU----------UCGG- -5' |
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| 31924 | 3' | -47.8 | NC_006940.1 | + | 15913 | 0.67 | 0.937422 |
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Target: 5'- cCAUGGCCUGUgAUAcCUGuguCACcGGCa -3' miRNA: 3'- -GUGCCGGAUA-UAUuGACu--GUGuUCGg -5' |
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| 31924 | 3' | -47.8 | NC_006940.1 | + | 12524 | 0.67 | 0.931646 |
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Target: 5'- uGCGGgCUuuu---CUGuACACAGGCCu -3' miRNA: 3'- gUGCCgGAuauauuGAC-UGUGUUCGG- -5' |
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| 31924 | 3' | -47.8 | NC_006940.1 | + | 10411 | 0.67 | 0.925548 |
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Target: 5'- -cCGGCCUcGUAUucugcCUGACGCGcuucGCCg -3' miRNA: 3'- guGCCGGA-UAUAuu---GACUGUGUu---CGG- -5' |
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| 31924 | 3' | -47.8 | NC_006940.1 | + | 25013 | 0.69 | 0.882231 |
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Target: 5'- uCACuGGCCgcgcUGUGUGagguuuuaaacAUUGACGCAAGCa -3' miRNA: 3'- -GUG-CCGG----AUAUAU-----------UGACUGUGUUCGg -5' |
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| 31924 | 3' | -47.8 | NC_006940.1 | + | 13309 | 0.73 | 0.637927 |
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Target: 5'- cCACGGCacgc-UGGCUGAUACGuGCCg -3' miRNA: 3'- -GUGCCGgauauAUUGACUGUGUuCGG- -5' |
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| 31924 | 3' | -47.8 | NC_006940.1 | + | 12245 | 0.74 | 0.578565 |
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Target: 5'- gGCGGaauguCUGgAUGACUGAUACAGGCUu -3' miRNA: 3'- gUGCCg----GAUaUAUUGACUGUGUUCGG- -5' |
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| 31924 | 3' | -47.8 | NC_006940.1 | + | 12557 | 1.13 | 0.002235 |
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Target: 5'- aCACGGCCUAUAUAACUGACACAAGCCc -3' miRNA: 3'- -GUGCCGGAUAUAUUGACUGUGUUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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