Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31925 | 3' | -62 | NC_006940.1 | + | 13934 | 0.66 | 0.299698 |
Target: 5'- gCCGCCgaGCUgGAGCGC-ACCUCaCAUu -3' miRNA: 3'- aGGCGGg-UGGgUUCGCGgUGGAG-GUG- -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 20488 | 0.66 | 0.285275 |
Target: 5'- -gCGCaCCACuuCCAcGCGCCuuACUUCCACc -3' miRNA: 3'- agGCG-GGUG--GGUuCGCGG--UGGAGGUG- -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 35516 | 0.67 | 0.264676 |
Target: 5'- --aGCCCAacggcaaUAGGCGCCGCCUUCGa -3' miRNA: 3'- aggCGGGUgg-----GUUCGCGGUGGAGGUg -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 28796 | 0.68 | 0.215638 |
Target: 5'- -aCGCCUAUUCuGGCGCCGCCaccugCCAg -3' miRNA: 3'- agGCGGGUGGGuUCGCGGUGGa----GGUg -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 25696 | 0.68 | 0.210087 |
Target: 5'- cCCGCCCACCgCAAuguuCGCUucgcuCCUCCGu -3' miRNA: 3'- aGGCGGGUGG-GUUc---GCGGu----GGAGGUg -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 17728 | 0.68 | 0.20466 |
Target: 5'- uUCCGgUaacagCACCCAGGUGCgGCC-CCGCc -3' miRNA: 3'- -AGGCgG-----GUGGGUUCGCGgUGGaGGUG- -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 16311 | 0.68 | 0.199355 |
Target: 5'- aCCGCgUACCUGAGCGUCAacaggacgCUCUACg -3' miRNA: 3'- aGGCGgGUGGGUUCGCGGUg-------GAGGUG- -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 19639 | 0.69 | 0.189103 |
Target: 5'- cCCGCCaCGCCgGAGCacGCCGCCcaauaCACu -3' miRNA: 3'- aGGCGG-GUGGgUUCG--CGGUGGag---GUG- -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 143 | 0.7 | 0.148528 |
Target: 5'- aUCCGCCUAUgacGGCGCCG-CUCCGCu -3' miRNA: 3'- -AGGCGGGUGgguUCGCGGUgGAGGUG- -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 37858 | 0.72 | 0.112863 |
Target: 5'- aCCGaCUCACCgGgggucAGCGCCGCCUUUACc -3' miRNA: 3'- aGGC-GGGUGGgU-----UCGCGGUGGAGGUG- -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 9597 | 0.76 | 0.052617 |
Target: 5'- uUUCGCCCGCCUgaauaaguuGAGCGCUACCaCCAUa -3' miRNA: 3'- -AGGCGGGUGGG---------UUCGCGGUGGaGGUG- -5' |
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31925 | 3' | -62 | NC_006940.1 | + | 19911 | 1.09 | 0.000125 |
Target: 5'- cUCCGCCCACCCAAGCGCCACCUCCACa -3' miRNA: 3'- -AGGCGGGUGGGUUCGCGGUGGAGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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