Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31926 | 3' | -55.6 | NC_006940.1 | + | 9497 | 0.66 | 0.632627 |
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Target: 5'- aAUCGCUCGuaACGUAAGUCAgGCGu-- -3' miRNA: 3'- gUAGCGGGC--UGCGUUUAGUgCGCcgu -5' |
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| 31926 | 3' | -55.6 | NC_006940.1 | + | 19353 | 0.66 | 0.61016 |
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Target: 5'- ---gGCauaaCGACGCGGua-GCGCGGCGc -3' miRNA: 3'- guagCGg---GCUGCGUUuagUGCGCCGU- -5' |
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| 31926 | 3' | -55.6 | NC_006940.1 | + | 6591 | 0.67 | 0.565539 |
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Target: 5'- --aCGCCCGcuucACGCGAcccgCAcCGCGGUAc -3' miRNA: 3'- guaGCGGGC----UGCGUUua--GU-GCGCCGU- -5' |
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| 31926 | 3' | -55.6 | NC_006940.1 | + | 19534 | 0.68 | 0.500433 |
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Target: 5'- -cUUGCUCGcCGU-AAUCAUGCGGCu -3' miRNA: 3'- guAGCGGGCuGCGuUUAGUGCGCCGu -5' |
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| 31926 | 3' | -55.6 | NC_006940.1 | + | 36983 | 0.68 | 0.500433 |
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Target: 5'- -uUCGCuCCGcGCGCGAGcaGgGCGGCAa -3' miRNA: 3'- guAGCG-GGC-UGCGUUUagUgCGCCGU- -5' |
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| 31926 | 3' | -55.6 | NC_006940.1 | + | 12156 | 0.68 | 0.455871 |
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Target: 5'- uCGUCGCCUuuaGACGUggGUacgaguucccaugaCGCGCGGUc -3' miRNA: 3'- -GUAGCGGG---CUGCGuuUA--------------GUGCGCCGu -5' |
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| 31926 | 3' | -55.6 | NC_006940.1 | + | 14753 | 0.71 | 0.350071 |
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Target: 5'- uCGUCGCCCuGuACGCGGuUCcacggaaacccucgaACGCGGCGg -3' miRNA: 3'- -GUAGCGGG-C-UGCGUUuAG---------------UGCGCCGU- -5' |
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| 31926 | 3' | -55.6 | NC_006940.1 | + | 25959 | 0.71 | 0.321843 |
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Target: 5'- aUAUCcaGCCCGGCGCGGAacguaUCaaGCGCGGUu -3' miRNA: 3'- -GUAG--CGGGCUGCGUUU-----AG--UGCGCCGu -5' |
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| 31926 | 3' | -55.6 | NC_006940.1 | + | 19773 | 0.75 | 0.190063 |
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Target: 5'- gGUCGCUCGAaGCc-GUCGCGCGGCc -3' miRNA: 3'- gUAGCGGGCUgCGuuUAGUGCGCCGu -5' |
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| 31926 | 3' | -55.6 | NC_006940.1 | + | 24897 | 1.08 | 0.000739 |
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Target: 5'- gCAUCGCCCGACGCAAAUCACGCGGCAu -3' miRNA: 3'- -GUAGCGGGCUGCGUUUAGUGCGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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