Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31927 | 3' | -50.5 | NC_006940.1 | + | 10695 | 0.66 | 0.860516 |
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Target: 5'- cUGGuGGUCguUGCCGcuUGUCUUAUCc -3' miRNA: 3'- aGCC-CCAGguACGGUuuGUAGAAUGG- -5' |
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| 31927 | 3' | -50.5 | NC_006940.1 | + | 15028 | 0.66 | 0.860516 |
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Target: 5'- gUCGGuGGUuaCCGccGCCGGAgGUCUcGCCc -3' miRNA: 3'- -AGCC-CCA--GGUa-CGGUUUgUAGAaUGG- -5' |
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| 31927 | 3' | -50.5 | NC_006940.1 | + | 32352 | 0.67 | 0.842666 |
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Target: 5'- -aGGGGgCCAcGCCAuGCGUaauagUUACCg -3' miRNA: 3'- agCCCCaGGUaCGGUuUGUAg----AAUGG- -5' |
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| 31927 | 3' | -50.5 | NC_006940.1 | + | 19099 | 0.67 | 0.842666 |
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Target: 5'- cUUGGGGUUCcgGCgCGAACGUUUaAgCg -3' miRNA: 3'- -AGCCCCAGGuaCG-GUUUGUAGAaUgG- -5' |
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| 31927 | 3' | -50.5 | NC_006940.1 | + | 2475 | 0.67 | 0.833367 |
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Target: 5'- gUGGuGGUCgGUGCCuGAAUAUCUguagACg -3' miRNA: 3'- aGCC-CCAGgUACGG-UUUGUAGAa---UGg -5' |
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| 31927 | 3' | -50.5 | NC_006940.1 | + | 1235 | 0.78 | 0.285931 |
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Target: 5'- gUCGuuGUCCGUGCCGAACGUUaccagUGCCg -3' miRNA: 3'- -AGCccCAGGUACGGUUUGUAGa----AUGG- -5' |
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| 31927 | 3' | -50.5 | NC_006940.1 | + | 26002 | 1.13 | 0.001116 |
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Target: 5'- gUCGGGGUCCAUGCCAAACAUCUUACCu -3' miRNA: 3'- -AGCCCCAGGUACGGUUUGUAGAAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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