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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31928 | 3' | -47.1 | NC_006940.1 | + | 5974 | 0.66 | 0.971339 |
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Target: 5'- uGCAAcCGGAacacuucagacguaACUGCCGUguGCACuccgcguuacguacgUCGAUGg -3' miRNA: 3'- -CGUU-GUCU--------------UGAUGGCA--UGUG---------------AGCUAC- -5' |
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| 31928 | 3' | -47.1 | NC_006940.1 | + | 35729 | 0.67 | 0.963827 |
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Target: 5'- aCAGCGGAA--ACCgGUACGC-CGAUGc -3' miRNA: 3'- cGUUGUCUUgaUGG-CAUGUGaGCUAC- -5' |
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| 31928 | 3' | -47.1 | NC_006940.1 | + | 15651 | 0.67 | 0.963827 |
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Target: 5'- gGUAACGcuGCUGCCGUAgGCUcCGGg- -3' miRNA: 3'- -CGUUGUcuUGAUGGCAUgUGA-GCUac -5' |
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| 31928 | 3' | -47.1 | NC_006940.1 | + | 35556 | 0.67 | 0.959805 |
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Target: 5'- cGCGgacGCGGggUUAUCG-ACAC-CGAUGu -3' miRNA: 3'- -CGU---UGUCuuGAUGGCaUGUGaGCUAC- -5' |
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| 31928 | 3' | -47.1 | NC_006940.1 | + | 33876 | 1.11 | 0.004024 |
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Target: 5'- gGCAACAGAACUACCGUACACUCGAUGc -3' miRNA: 3'- -CGUUGUCUUGAUGGCAUGUGAGCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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