Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31930 | 3' | -54.8 | NC_006940.1 | + | 34206 | 0.66 | 0.673542 |
Target: 5'- aUAGUCGCCaauaUCGCGGCUGCCaaUGGu -3' miRNA: 3'- cAUUAGUGGa---AGUGCCGGUGGcgGCU- -5' |
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31930 | 3' | -54.8 | NC_006940.1 | + | 10266 | 0.66 | 0.655529 |
Target: 5'- gGUGGUCuucuuguugaugcuCCggaaacagcCGCGGCCACCGCUGc -3' miRNA: 3'- -CAUUAGu-------------GGaa-------GUGCCGGUGGCGGCu -5' |
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31930 | 3' | -54.8 | NC_006940.1 | + | 17732 | 0.66 | 0.651015 |
Target: 5'- gGUAAcagCACCcaggUGCGGCC-CCGCCa- -3' miRNA: 3'- -CAUUa--GUGGaa--GUGCCGGuGGCGGcu -5' |
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31930 | 3' | -54.8 | NC_006940.1 | + | 5245 | 0.66 | 0.639719 |
Target: 5'- uUAAaCGCUggUGCGGCC-CCGCCGc -3' miRNA: 3'- cAUUaGUGGaaGUGCCGGuGGCGGCu -5' |
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31930 | 3' | -54.8 | NC_006940.1 | + | 5838 | 0.66 | 0.617114 |
Target: 5'- gGUGAUgCACCaaaaAUGuaCACCGCCGAa -3' miRNA: 3'- -CAUUA-GUGGaag-UGCcgGUGGCGGCU- -5' |
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31930 | 3' | -54.8 | NC_006940.1 | + | 20569 | 0.67 | 0.605828 |
Target: 5'- -cAGUaCAgUUUCGCgaGGCCccGCCGCCGAa -3' miRNA: 3'- caUUA-GUgGAAGUG--CCGG--UGGCGGCU- -5' |
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31930 | 3' | -54.8 | NC_006940.1 | + | 716 | 0.69 | 0.485189 |
Target: 5'- -------gUUUCACGGCUGCCGUCGAg -3' miRNA: 3'- cauuagugGAAGUGCCGGUGGCGGCU- -5' |
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31930 | 3' | -54.8 | NC_006940.1 | + | 14352 | 0.69 | 0.484139 |
Target: 5'- ---cUUugCUUCGCGGCCGauggcuuCCGCCu- -3' miRNA: 3'- cauuAGugGAAGUGCCGGU-------GGCGGcu -5' |
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31930 | 3' | -54.8 | NC_006940.1 | + | 17494 | 0.7 | 0.386331 |
Target: 5'- cGUGGUCACCcccgGCGGUauaggacauaGCCGCCGAa -3' miRNA: 3'- -CAUUAGUGGaag-UGCCGg---------UGGCGGCU- -5' |
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31930 | 3' | -54.8 | NC_006940.1 | + | 36319 | 1.09 | 0.000769 |
Target: 5'- gGUAAUCACCUUCACGGCCACCGCCGAg -3' miRNA: 3'- -CAUUAGUGGAAGUGCCGGUGGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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