Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31931 | 3' | -55.3 | NC_006940.1 | + | 2454 | 0.66 | 0.627185 |
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Target: 5'- gCUACGGUuaauGCUGuacCCGUGGUGGuCg -3' miRNA: 3'- -GAUGCUAu---CGGCuuaGGCGCCGCCuG- -5' |
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| 31931 | 3' | -55.3 | NC_006940.1 | + | 26484 | 0.66 | 0.627185 |
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Target: 5'- -cGCGAUugcucaaCCGGuuGUCCGCGGCaaucgugcGGACa -3' miRNA: 3'- gaUGCUAuc-----GGCU--UAGGCGCCG--------CCUG- -5' |
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| 31931 | 3' | -55.3 | NC_006940.1 | + | 11091 | 0.67 | 0.571372 |
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Target: 5'- -cGCGGUAGCCGAG-CU--GGUGGAUa -3' miRNA: 3'- gaUGCUAUCGGCUUaGGcgCCGCCUG- -5' |
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| 31931 | 3' | -55.3 | NC_006940.1 | + | 24825 | 0.67 | 0.549344 |
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Target: 5'- -aGCGAUAuGCCGcguGAUuuGCGuCGGGCg -3' miRNA: 3'- gaUGCUAU-CGGC---UUAggCGCcGCCUG- -5' |
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| 31931 | 3' | -55.3 | NC_006940.1 | + | 14954 | 0.71 | 0.360193 |
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Target: 5'- ---aGAUGGgCGAGaccUCCGCGGCGGu- -3' miRNA: 3'- gaugCUAUCgGCUU---AGGCGCCGCCug -5' |
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| 31931 | 3' | -55.3 | NC_006940.1 | + | 38042 | 1.1 | 0.000598 |
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Target: 5'- uCUACGAUAGCCGAAUCCGCGGCGGACg -3' miRNA: 3'- -GAUGCUAUCGGCUUAGGCGCCGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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