Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31936 | 5' | -49.4 | NC_006940.1 | + | 401 | 0.66 | 0.916536 |
|
Target: 5'- aGGGUAUGcuggauGCCCGUCccGGUUAcGACu -3' miRNA: 3'- gCCCAUAU------UGGGCGGcuUCAAUcUUG- -5' |
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| 31936 | 5' | -49.4 | NC_006940.1 | + | 2131 | 1.1 | 0.00258 |
|
Target: 5'- gCGGGUAUAACCCGCCGAAGUUAGAACc -3' miRNA: 3'- -GCCCAUAUUGGGCGGCUUCAAUCUUG- -5' |
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| 31936 | 5' | -49.4 | NC_006940.1 | + | 23268 | 0.66 | 0.922971 |
|
Target: 5'- cCGGGg---GCgCGUCGGAGU-GGGACa -3' miRNA: 3'- -GCCCauauUGgGCGGCUUCAaUCUUG- -5' |
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| 31936 | 5' | -49.4 | NC_006940.1 | + | 31436 | 0.77 | 0.378287 |
|
Target: 5'- aGGGUAUAGCaCCGCCGAAucccGGAAUu -3' miRNA: 3'- gCCCAUAUUG-GGCGGCUUcaa-UCUUG- -5' |
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| 31936 | 5' | -49.4 | NC_006940.1 | + | 32109 | 0.7 | 0.720182 |
|
Target: 5'- aGGGUcuu-CCCGCUGAAGUUA--ACg -3' miRNA: 3'- gCCCAuauuGGGCGGCUUCAAUcuUG- -5' |
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| 31936 | 5' | -49.4 | NC_006940.1 | + | 34239 | 0.68 | 0.845152 |
|
Target: 5'- uGGGUGUcuauaAACCgCaCCGAGGUU-GAGCg -3' miRNA: 3'- gCCCAUA-----UUGG-GcGGCUUCAAuCUUG- -5' |
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| 31936 | 5' | -49.4 | NC_006940.1 | + | 39989 | 0.7 | 0.731463 |
|
Target: 5'- gGGGUGgauucuUCUGCCGuGGGUUGGGGCu -3' miRNA: 3'- gCCCAUauu---GGGCGGC-UUCAAUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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