miRNA: 3'- aCCCAuCGUag-CUACUUAcGUGGACg -5'
miRNA: 3'- aCCCAUCGUAG-CUACUU--AC-GUGGacg -5'
miRNA: 3'- aCcCAUCGUAGCUACUUAcg-UGGACg -5'
miRNA: 3'- -ACCCAUCGUAGCUACUUACGUGGACG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31937 | 5' | -51.1 | NC_006940.1 | + | 27842 | 0.66 | 0.80973 |
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Target: 5'- gGGGU-GCAauaGAUGAGUaCGCCUGa -3' miRNA: 3'- aCCCAuCGUag-CUACUUAcGUGGACg -5' |
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| 31937 | 5' | -51.1 | NC_006940.1 | + | 38228 | 0.69 | 0.689686 |
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Target: 5'- cGGGUAGCGUCaGGUGAGuuUGuCGCUg-- -3' miRNA: 3'- aCCCAUCGUAG-CUACUU--AC-GUGGacg -5' |
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| 31937 | 5' | -51.1 | NC_006940.1 | + | 10877 | 0.72 | 0.483544 |
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Target: 5'- cGuGUAGCAUCGGUGAAUaaaGCCUGg -3' miRNA: 3'- aCcCAUCGUAGCUACUUAcg-UGGACg -5' |
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| 31937 | 5' | -51.1 | NC_006940.1 | + | 4624 | 1.13 | 0.000814 |
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Target: 5'- cUGGGUAGCAUCGAUGAAUGCACCUGCg -3' miRNA: 3'- -ACCCAUCGUAGCUACUUACGUGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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