Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31939 | 5' | -60.1 | NC_006940.1 | + | 37620 | 0.66 | 0.397904 |
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Target: 5'- aGCAGCAUugUCCGCGGaauCGCCuACa -3' miRNA: 3'- gCGUUGUGcuGGGCGCCgu-GCGGcUG- -5' |
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| 31939 | 5' | -60.1 | NC_006940.1 | + | 9117 | 0.66 | 0.397904 |
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Target: 5'- gCGCAuuuacGCACaACCCGCGGUAuCGUCu-- -3' miRNA: 3'- -GCGU-----UGUGcUGGGCGCCGU-GCGGcug -5' |
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| 31939 | 5' | -60.1 | NC_006940.1 | + | 33938 | 0.66 | 0.392527 |
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Target: 5'- --uGGCGCGcACCCGCacccgugacacaaccGGCAgGUCGGCg -3' miRNA: 3'- gcgUUGUGC-UGGGCG---------------CCGUgCGGCUG- -5' |
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| 31939 | 5' | -60.1 | NC_006940.1 | + | 25047 | 0.68 | 0.307118 |
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Target: 5'- aCGCaAGCAUGGCUaaggauaaaCGCGGUaagGCGCUGAUa -3' miRNA: 3'- -GCG-UUGUGCUGG---------GCGCCG---UGCGGCUG- -5' |
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| 31939 | 5' | -60.1 | NC_006940.1 | + | 26474 | 0.68 | 0.302649 |
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Target: 5'- gCGCAcagaACGCGAuugcucaaccgguugUCCGCGGCAaucgUGCgGACa -3' miRNA: 3'- -GCGU----UGUGCU---------------GGGCGCCGU----GCGgCUG- -5' |
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| 31939 | 5' | -60.1 | NC_006940.1 | + | 13320 | 0.69 | 0.239109 |
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Target: 5'- -cUAAUAgaGACCCaCGGCACGCUGGCu -3' miRNA: 3'- gcGUUGUg-CUGGGcGCCGUGCGGCUG- -5' |
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| 31939 | 5' | -60.1 | NC_006940.1 | + | 16364 | 0.74 | 0.116034 |
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Target: 5'- cCGCAGCGCGugCgGCuaccuGGCACGCCu-- -3' miRNA: 3'- -GCGUUGUGCugGgCG-----CCGUGCGGcug -5' |
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| 31939 | 5' | -60.1 | NC_006940.1 | + | 6595 | 0.78 | 0.06071 |
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Target: 5'- cCGCuucACGCGACCCgcaccGCGGUACGCUGAa -3' miRNA: 3'- -GCGu--UGUGCUGGG-----CGCCGUGCGGCUg -5' |
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| 31939 | 5' | -60.1 | NC_006940.1 | + | 14405 | 1.09 | 0.000225 |
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Target: 5'- gCGCAACACGACCCGCGGCACGCCGACg -3' miRNA: 3'- -GCGUUGUGCUGGGCGCCGUGCGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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