Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31941 | 5' | -59 | NC_006940.1 | + | 35502 | 0.66 | 0.471488 |
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Target: 5'- -aACCCCG-CGUC-CGCGCGAAcguugcggacgugauGCCa -3' miRNA: 3'- acUGGGGCgGUAGcGCGCGCUU---------------UGGc -5' |
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| 31941 | 5' | -59 | NC_006940.1 | + | 40312 | 0.66 | 0.4675 |
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Target: 5'- gUGACCaacaccuuucuUCGCCAggCGCGCagaaCGAAGCUGa -3' miRNA: 3'- -ACUGG-----------GGCGGUa-GCGCGc---GCUUUGGC- -5' |
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| 31941 | 5' | -59 | NC_006940.1 | + | 19171 | 0.66 | 0.428621 |
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Target: 5'- cUGGCCUaUGCCGaCGUggacaGCGCGAcGCCGg -3' miRNA: 3'- -ACUGGG-GCGGUaGCG-----CGCGCUuUGGC- -5' |
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| 31941 | 5' | -59 | NC_006940.1 | + | 18925 | 0.69 | 0.301471 |
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Target: 5'- aGGCCCUGaCguUCGCGCGuUGggGCUu -3' miRNA: 3'- aCUGGGGC-GguAGCGCGC-GCuuUGGc -5' |
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| 31941 | 5' | -59 | NC_006940.1 | + | 813 | 0.73 | 0.170458 |
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Target: 5'- --uCCCCGCCGUaaCGCGCGAcGCCc -3' miRNA: 3'- acuGGGGCGGUAgcGCGCGCUuUGGc -5' |
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| 31941 | 5' | -59 | NC_006940.1 | + | 36909 | 0.74 | 0.129716 |
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Target: 5'- cGGCCagguugCCGCCcucUCGCGCGCGGAGCg- -3' miRNA: 3'- aCUGG------GGCGGu--AGCGCGCGCUUUGgc -5' |
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| 31941 | 5' | -59 | NC_006940.1 | + | 31554 | 1.08 | 0.000392 |
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Target: 5'- aUGACCCCGCCAUCGCGCGCGAAACCGc -3' miRNA: 3'- -ACUGGGGCGGUAGCGCGCGCUUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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