Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31943 | 5' | -59.4 | NC_006940.1 | + | 38783 | 0.66 | 0.42054 |
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Target: 5'- cGCcuuauAGGCUgcgGCGGCUacaACGGCAGcCAGu -3' miRNA: 3'- cCGc----UCUGGa--CGCCGA---UGCCGUC-GUC- -5' |
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| 31943 | 5' | -59.4 | NC_006940.1 | + | 30397 | 0.68 | 0.317073 |
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Target: 5'- --aGAGGuuUuaCGGCUACGGCAGCAa -3' miRNA: 3'- ccgCUCUggAc-GCCGAUGCCGUCGUc -5' |
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| 31943 | 5' | -59.4 | NC_006940.1 | + | 3929 | 0.68 | 0.294393 |
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Target: 5'- uGGCGG----UGCGGCUACuGUAGCGGa -3' miRNA: 3'- -CCGCUcuggACGCCGAUGcCGUCGUC- -5' |
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| 31943 | 5' | -59.4 | NC_006940.1 | + | 34033 | 0.68 | 0.290012 |
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Target: 5'- cGGCGucacuauuuacgccGACCUGcCGGUUgugucACGGguGCGGg -3' miRNA: 3'- -CCGCu-------------CUGGAC-GCCGA-----UGCCguCGUC- -5' |
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| 31943 | 5' | -59.4 | NC_006940.1 | + | 2924 | 0.69 | 0.252868 |
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Target: 5'- aGGUGAuGGCC-GCGGUugagUACGGC-GCGGg -3' miRNA: 3'- -CCGCU-CUGGaCGCCG----AUGCCGuCGUC- -5' |
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| 31943 | 5' | -59.4 | NC_006940.1 | + | 5332 | 0.7 | 0.233976 |
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Target: 5'- uGGCGGGGCUUcCGGgaAUaaaGGCGGCGGg -3' miRNA: 3'- -CCGCUCUGGAcGCCgaUG---CCGUCGUC- -5' |
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| 31943 | 5' | -59.4 | NC_006940.1 | + | 14958 | 0.71 | 0.179425 |
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Target: 5'- gGGCGAGACCUccGCGGCgguaaccaccgACGccauguaCAGCAGg -3' miRNA: 3'- -CCGCUCUGGA--CGCCGa----------UGCc------GUCGUC- -5' |
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| 31943 | 5' | -59.4 | NC_006940.1 | + | 3599 | 0.77 | 0.069158 |
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Target: 5'- gGGUGAuagccuGACCUGCGGUUACcGCAGUAGc -3' miRNA: 3'- -CCGCU------CUGGACGCCGAUGcCGUCGUC- -5' |
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| 31943 | 5' | -59.4 | NC_006940.1 | + | 36956 | 1.09 | 0.000247 |
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Target: 5'- uGGCGAGACCUGCGGCUACGGCAGCAGc -3' miRNA: 3'- -CCGCUCUGGACGCCGAUGCCGUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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