Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31945 | 3' | -55.2 | NC_006940.1 | + | 10178 | 0.66 | 0.559099 |
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Target: 5'- ----cGGCaGCGGUGGCc---GCGGCu -3' miRNA: 3'- aauauCCGaCGCCGCCGauguUGCCG- -5' |
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| 31945 | 3' | -55.2 | NC_006940.1 | + | 16532 | 0.67 | 0.493086 |
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Target: 5'- --cUGGGCUGCGccgaCGGCUAUAgagccauuGCGGa -3' miRNA: 3'- aauAUCCGACGCc---GCCGAUGU--------UGCCg -5' |
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| 31945 | 3' | -55.2 | NC_006940.1 | + | 19150 | 0.68 | 0.46143 |
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Target: 5'- -gGUAGaGCUuaccGUGGCGGUacaaGCGGCGGUa -3' miRNA: 3'- aaUAUC-CGA----CGCCGCCGa---UGUUGCCG- -5' |
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| 31945 | 3' | -55.2 | NC_006940.1 | + | 16125 | 0.68 | 0.449069 |
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Target: 5'- gUGUAGGCaaaggucUGUGGCGuGCgcacucuUACGACGGg -3' miRNA: 3'- aAUAUCCG-------ACGCCGC-CG-------AUGUUGCCg -5' |
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| 31945 | 3' | -55.2 | NC_006940.1 | + | 7907 | 0.69 | 0.399636 |
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Target: 5'- ---gAGGCgcgaaaguUGCGGCGGCccauacuuguuCGGCGGCc -3' miRNA: 3'- aauaUCCG--------ACGCCGCCGau---------GUUGCCG- -5' |
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| 31945 | 3' | -55.2 | NC_006940.1 | + | 6404 | 0.74 | 0.193253 |
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Target: 5'- ----cGGCUGCcGUGGCUACcGCGGUu -3' miRNA: 3'- aauauCCGACGcCGCCGAUGuUGCCG- -5' |
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| 31945 | 3' | -55.2 | NC_006940.1 | + | 3892 | 0.75 | 0.177658 |
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Target: 5'- ----cGGCUGCcGCGGCUGCAccacuacCGGCa -3' miRNA: 3'- aauauCCGACGcCGCCGAUGUu------GCCG- -5' |
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| 31945 | 3' | -55.2 | NC_006940.1 | + | 38786 | 1.1 | 0.000466 |
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Target: 5'- cUUAUAGGCUGCGGCGGCUACAACGGCa -3' miRNA: 3'- -AAUAUCCGACGCCGCCGAUGUUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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