Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31980 | 3' | -55 | NC_006948.1 | + | 5257 | 0.69 | 0.097006 |
Target: 5'- cCGUGGuAUGGUUgaGGCCAU-CCGCGGg -3' miRNA: 3'- -GCGCC-UACCGGagUUGGUAuGGUGCC- -5' |
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31980 | 3' | -55 | NC_006948.1 | + | 5288 | 1.12 | 0.000026 |
Target: 5'- cCGCGGAUGGCCUCAACCAUACCACGGg -3' miRNA: 3'- -GCGCCUACCGGAGUUGGUAUGGUGCC- -5' |
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31980 | 3' | -55 | NC_006948.1 | + | 2675 | 0.67 | 0.149043 |
Target: 5'- aCGgGGgcGGCCUCAGgC---UCACGGg -3' miRNA: 3'- -GCgCCuaCCGGAGUUgGuauGGUGCC- -5' |
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31980 | 3' | -55 | NC_006948.1 | + | 3270 | 0.66 | 0.17512 |
Target: 5'- uGCGGAUccGCCUCAuauAUCugGCCAUGGc -3' miRNA: 3'- gCGCCUAc-CGGAGU---UGGuaUGGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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