miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31982 3' -54.7 NC_006948.1 + 1699 0.66 0.157871
Target:  5'- cACGGguUCG-CGGCGCAUuucgcggAGCUUGGCg -3'
miRNA:   3'- -UGUUguGGCgGUCGUGUA-------UCGAGCCG- -5'
31982 3' -54.7 NC_006948.1 + 4905 0.67 0.134155
Target:  5'- uGCcAUACUGCCgGGCAUcauuUGGCUgGGCc -3'
miRNA:   3'- -UGuUGUGGCGG-UCGUGu---AUCGAgCCG- -5'
31982 3' -54.7 NC_006948.1 + 5390 0.68 0.113374
Target:  5'- gGCGAgugUGCCGCC-GCACcUAGUgUCGGCc -3'
miRNA:   3'- -UGUU---GUGGCGGuCGUGuAUCG-AGCCG- -5'
31982 3' -54.7 NC_006948.1 + 3232 0.68 0.105936
Target:  5'- aGCAugACCGUCugcuGCAUgaaggaggcgAUAGUUUGGCc -3'
miRNA:   3'- -UGUugUGGCGGu---CGUG----------UAUCGAGCCG- -5'
31982 3' -54.7 NC_006948.1 + 5513 0.68 0.098957
Target:  5'- cACGACuuGCCGCCGGCAUcgugGGCcgcccaaGGCu -3'
miRNA:   3'- -UGUUG--UGGCGGUCGUGua--UCGag-----CCG- -5'
31982 3' -54.7 NC_006948.1 + 1427 1.11 0.000025
Target:  5'- gACAACACCGCCAGCACAUAGCUCGGCc -3'
miRNA:   3'- -UGUUGUGGCGGUCGUGUAUCGAGCCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.