Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31982 | 5' | -58.6 | NC_006948.1 | + | 938 | 0.66 | 0.101498 |
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Target: 5'- cCCGCCCccUCCAC-UC-CACCUu-CGCg -3' miRNA: 3'- -GGCGGG--AGGUGuAGuGUGGGcuGCGa -5' |
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| 31982 | 5' | -58.6 | NC_006948.1 | + | 2241 | 0.66 | 0.098234 |
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Target: 5'- aCGCCa-CC-CAUCGCAaCCGGCGUg -3' miRNA: 3'- gGCGGgaGGuGUAGUGUgGGCUGCGa -5' |
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| 31982 | 5' | -58.6 | NC_006948.1 | + | 4304 | 0.66 | 0.09507 |
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Target: 5'- gCgGaCCCUCUgcaGCAUCGCggGCCC-ACGCUg -3' miRNA: 3'- -GgC-GGGAGG---UGUAGUG--UGGGcUGCGA- -5' |
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| 31982 | 5' | -58.6 | NC_006948.1 | + | 163 | 0.67 | 0.070637 |
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Target: 5'- cUCGCCCgagccacCCGCAUCA-ACCCGuuCGCc -3' miRNA: 3'- -GGCGGGa------GGUGUAGUgUGGGCu-GCGa -5' |
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| 31982 | 5' | -58.6 | NC_006948.1 | + | 4982 | 0.68 | 0.063926 |
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Target: 5'- cCCGCCCcCCugAUUGCACCau-CGUUg -3' miRNA: 3'- -GGCGGGaGGugUAGUGUGGgcuGCGA- -5' |
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| 31982 | 5' | -58.6 | NC_006948.1 | + | 2501 | 0.7 | 0.042744 |
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Target: 5'- aCCGCCCuUUCGCG-CGC-CCgCGACGCc -3' miRNA: 3'- -GGCGGG-AGGUGUaGUGuGG-GCUGCGa -5' |
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| 31982 | 5' | -58.6 | NC_006948.1 | + | 253 | 0.71 | 0.036102 |
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Target: 5'- gCCGCCCgcuuaCACA-CGCACgCCGcCGCUa -3' miRNA: 3'- -GGCGGGag---GUGUaGUGUG-GGCuGCGA- -5' |
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| 31982 | 5' | -58.6 | NC_006948.1 | + | 5174 | 0.71 | 0.03598 |
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Target: 5'- aCCGCCCUCgAC-UCGCucgggccGCCCGACcCUc -3' miRNA: 3'- -GGCGGGAGgUGuAGUG-------UGGGCUGcGA- -5' |
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| 31982 | 5' | -58.6 | NC_006948.1 | + | 4631 | 0.77 | 0.011327 |
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Target: 5'- aUCGCgCUCaCGCGUCACACCCGGCu-- -3' miRNA: 3'- -GGCGgGAG-GUGUAGUGUGGGCUGcga -5' |
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| 31982 | 5' | -58.6 | NC_006948.1 | + | 1463 | 1.1 | 0.000015 |
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Target: 5'- cCCGCCCUCCACAUCACACCCGACGCUg -3' miRNA: 3'- -GGCGGGAGGUGUAGUGUGGGCUGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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